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贝壳英

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[求助] 把下面的英文摘要翻译成中文

A novel colorimetric triple-helix molecular switch aptasensor for ul- trasensitive detection of tetracycline
a b s t r a c t
Detection methods of antibiotic residues in blood serum and animal derived foods are of great interest. In this study a    colorimetric aptasensor was designed for sensitive, selective   and fast detection of tetracycline based on triple-helix    molecular switch(THMS) and gold nanoparticles (AuNPs). As a biosensor, THMS shows distinct advantages including high     stability, sensitivity and preserving the selectivity and affinity  of the original         aptamer. In the absence of tetracycline, THMS is stable, leading to the aggregation of AuNPs by salt and anobvious color change from red to blue.   In the presence of   tetracycline, aptamer binds toitstarget, signal transduction     probe(STP) leaves the THMS and adsorbs on the surface of  AuNPs. So the well-dispersed AuNPs remain stable against    salt-induced aggregation with a red color. The presented     aptasensor showed high selectivity toward tetracyclines with a limit of detection as low as 266 pM for tetracycline. The    designed aptasensor was successfully applied to detect              tetracycline in serum and milk.
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现在，青春是用来奋斗的；未来，青春是用来怀念的。
1楼 2016-12-14 18:42:30
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jiangguofeng

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贝壳英(fjtony163代发): 金币+10, 代发 2017-01-19 15:31:44
fjtony163: 翻译EPI+1, 代发 2017-01-19 15:31:52
一种新型的用于四环素超灵敏检测的比色三重螺旋分子开关适体传感器
摘要
血清和动物来源食品中抗菌素残留物的检测方法将会受到极大的关注。于这项研究中，基于三重螺旋分子开关(THMS)和金纳米粒子(AuNPs)设计了一种用于灵敏快速、有选择性的四环素检测方法的比色适体传感器。作为一种生物传感器, THMS显示出明显的优点，包括高稳定性和高灵敏度并能保持原适体的选择性和亲合力。在不存在四环素的情况下, THMS是稳定的,通过盐引起AuNPs聚集和一个由红到蓝明显的变色。 在有四环素存在下,适体结合其靶标,信号转导探针( STP)离开THMS并吸附于AuNPs的表面上。因此充分分散的AuNPs对于盐诱导的聚集保持稳定并带有一种红颜色。提出的这种适体传感器显示出对四环素的高选择性，四环素的检测极限低至266 pM。设计的这种适体传感器成功地应用到了血清和牛奶中四环素的检测当中。
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2楼2016-12-17 12:56:54
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Abstract: In this paper, 2-chloro-quinoline and phenylboronic acid were used as starting materials, and the main ligand 2-phenylquinoline (pq) was obtained by the reaction of tetrakis (triphenylphosphine) palladium as a catalyst. Then pq was reacted with iridium trichloride (Pq) 2Ir (μ-Cl2) Ir (pq) 2 was synthesized by the reaction of 2-amino-2-methyl-2-propanol with ethylene glycol monoethylether and then acetylation reaction was carried out under alkaline conditions. 2-phenylquinoline-C2, N ') (acetylacetonato) iridium (III) Ir (pq) 2 (acac). The structures of the compounds were characterized by elemental analysis, IR, 1H NMR, 13C NMR, mass spectroscopy and single crystal X-ray diffraction. Their photophysical properties were characterized by UV-Vis absorption spectra and photoluminescence spectra. test. The results show that Ir (pq) 2 (acac) is an electrically neutral octahedral complex with an average Ir-O bond length of 0.21741 (18) nm and an Ir-C bond length of 0.1983 (3) The average length of the N bond is 0.2079 (2) nm, and the strong red emission occurs at 600nm. The synthetic yield is more than 95%. This method is suitable for small batch preparation of Ir (pq) 2 (acac).
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3楼2016-12-17 16:22:38
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是非对错

铁杆木虫 (正式写手)
不好意思，回错贴了
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只有想不到，没有做不到
4楼2016-12-17 16:23:25
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贝壳英

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引用回帖:
2楼: Originally posted by jiangguofeng at 2016-12-17 12:56:54
一种新型的用于四环素超灵敏检测的比色三重螺旋分子开关适体传感器
摘要
血清和动物来源食品中抗菌素残留物的检测方法将会受到极大的关注。于这项研究中，基于三重螺旋分子开关(THMS)和金纳米粒子(AuNPs)设计了一 ...

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现在，青春是用来奋斗的；未来，青春是用来怀念的。
5楼2016-12-18 07:31:05
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贝壳英

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引用回帖:
2楼: Originally posted by jiangguofeng at 2016-12-17 12:56:54
一种新型的用于四环素超灵敏检测的比色三重螺旋分子开关适体传感器
摘要
血清和动物来源食品中抗菌素残留物的检测方法将会受到极大的关注。于这项研究中，基于三重螺旋分子开关(THMS)和金纳米粒子(AuNPs)设计了一 ...

1.Introduction
Tetracyclines are broad-spectrum antibiotics which are used in human and veterinary therapy (Gan et al. 2014; Zhao and   Wang 2013). Excessive use of tetracyclines in veterinary     medicine as antibiotics and growth promotors could lead to  antibiotic residues in food products(Asadollahi-Baboli and     Mani-Varnosfaderani 2014; Tan et al.2013b). Residues of      tetracyclines in food products could result in undesirable side effects on human health and emergence of bacterial         resistance to antibiotics in both veterinary and human(Moreiraet al.2010; Tan et al.2013b). Allergic reactions, yellowing of   teeth and liver damage are among side effects of tetracyclines(Mu et al.2012).
   Therefore,selective,fast and sensitive methods for detectionof tetracyclines are of great interest.Current methods for     detection of tetracyclines are: immunoassays(Aga et al.2003;   Jeon and Rhee Paeng2008), high performance liquid         chromatography(HPLC) (Cinquina et al.2003; Shariati et al.2009), microbiological methods (Idowu et al.2010; Kurittu et al.2000), chemiluminescence (Townshend et al.2005) and capillary  electrophoresis(CE)(Kowalski 2008; Tong et al.2009). Generally  these approaches require sophisticated and expensive         instruments and need lengthy turnaround time(Chen et al.2014; Tan et al.2013b).
Aptamers are single-stranded oligonucleotides,obtained by an in vitro process named SELEX(systematic evolution of      ligands by exponential enrichment)(Lian et al.2015). They react to their targets ranging from small substances to proteins   and even cells with high specificity and affinity (Huang et al.2014; Zhao et al. 2015). Aptamers possess advantages over    antibodies like low cost, excellent thermal stability,ease of    production and modification, and lack of toxicity and         immunogenicity(Bai et al.2013; Lian et al. 2015; Luo et al.2014; Shan et al.2014). Owing to these unique properties,      aptamers have attracted substantial attentions for application  in biosensors(Evtugyn et al.2013; Taghdisi et al.2014).
Recently gold nanoparticles(AuNPs)have been broadly used in the development of colorimetric and fluorescence        quenching aptasensors, because of their unique characteristics such as chemical stability,high sensitivity,ease of synthesis and extremely high absorption coefficient (Gopinath et al.2014; Liu et al.2014; Tan et al. 2013a; Zhang et al. 2013).
Colorimetric assay is a common technique for analytical  applications, since the target recognition event can be       determined only by the naked eye (Gopinath et al. 2014).
    In this study a colorimetric triple-helix molecular switch (THMS) system was designed for the first time for detection of tetracyclines based on aptamer and AuNPs.THMS presents    unique advantages over known double-helix DNA molecular   switches and molecular beacon-based signaling aptamers      including high sensitivity and stability and preserving the     specificity and affinity of the original aptamer (Zheng et al.2011). THMS system usually consists of a label-free target specific aptamer sequence with two arm segments and a          dual-labeled oligonucleotide as a signal transduction probe(STP)(Verdian-Doghaei et al.2014; Zheng et al. 2011), but in this   study a label-free STP was used which is economical and    does not need any special instrument.In this project a       truncated 8-mer ssDNA aptamer,which selectively binds to    different tetracyclines(Kwon et al.2014), was used as targeting agent.
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现在，青春是用来奋斗的；未来，青春是用来怀念的。
6楼2016-12-18 08:23:08
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贝壳英

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引用回帖:
2楼: Originally posted by jiangguofeng at 2016-12-17 12:56:54
一种新型的用于四环素超灵敏检测的比色三重螺旋分子开关适体传感器
摘要
血清和动物来源食品中抗菌素残留物的检测方法将会受到极大的关注。于这项研究中，基于三重螺旋分子开关(THMS)和金纳米粒子(AuNPs)设计了一 ...

1.Introduction
Tetracyclines are broad-spectrum antibiotics which are used in human and veterinary therapy (Gan et al. 2014; Zhao and   Wang 2013). Excessive use of tetracyclines in veterinary     medicine as antibiotics and growth promotors could lead to  antibiotic residues in food products(Asadollahi-Baboli and     Mani-Varnosfaderani 2014; Tan et al.2013b). Residues of      tetracyclines in food products could result in undesirable side effects on human health and emergence of bacterial         resistance to antibiotics in both veterinary and human(Moreiraet al.2010; Tan et al.2013b). Allergic reactions, yellowing of   teeth and liver damage are among side effects of tetracyclines(Mu et al.2012).
   Therefore,selective,fast and sensitive methods for detectionof tetracyclines are of great interest.Current methods for     detection of tetracyclines are: immunoassays(Aga et al.2003;   Jeon and Rhee Paeng2008), high performance liquid         chromatography(HPLC) (Cinquina et al.2003; Shariati et al.2009), microbiological methods (Idowu et al.2010; Kurittu et al.2000), chemiluminescence (Townshend et al.2005) and capillary  electrophoresis(CE)(Kowalski 2008; Tong et al.2009). Generally  these approaches require sophisticated and expensive         instruments and need lengthy turnaround time(Chen et al.2014; Tan et al.2013b).
Aptamers are single-stranded oligonucleotides,obtained by an in vitro process named SELEX(systematic evolution of      ligands by exponential enrichment)(Lian et al.2015). They react to their targets ranging from small substances to proteins   and even cells with high specificity and affinity (Huang et al.2014; Zhao et al. 2015). Aptamers possess advantages over    antibodies like low cost, excellent thermal stability,ease of    production and modification, and lack of toxicity and         immunogenicity(Bai et al.2013; Lian et al. 2015; Luo et al.2014; Shan et al.2014). Owing to these unique properties,      aptamers have attracted substantial attentions for application  in biosensors(Evtugyn et al.2013; Taghdisi et al.2014).
Recently gold nanoparticles(AuNPs)have been broadly used in the development of colorimetric and fluorescence        quenching aptasensors, because of their unique characteristics such as chemical stability,high sensitivity,ease of synthesis and extremely high absorption coefficient (Gopinath et al.2014; Liu et al.2014; Tan et al. 2013a; Zhang et al. 2013).
Colorimetric assay is a common technique for analytical  applications, since the target recognition event can be       determined only by the naked eye (Gopinath et al. 2014).
    In this study a colorimetric triple-helix molecular switch (THMS) system was designed for the first time for detection of tetracyclines based on aptamer and AuNPs.THMS presents    unique advantages over known double-helix DNA molecular   switches and molecular beacon-based signaling aptamers      including high sensitivity and stability and preserving the     specificity and affinity of the original aptamer (Zheng et al.2011). THMS system usually consists of a label-free target specific aptamer sequence with two arm segments and a          dual-labeled oligonucleotide as a signal transduction probe(STP)(Verdian-Doghaei et al.2014; Zheng et al. 2011), but in this   study a label-free STP was used which is economical and    does not need any special instrument.In this project a       truncated 8-mer ssDNA aptamer,which selectively binds to    different tetracyclines(Kwon et al.2014), was used as targeting agent.
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现在，青春是用来奋斗的；未来，青春是用来怀念的。
7楼2016-12-18 08:23:44
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jiangguofeng

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1.引言
四环素是一种人用和兽用治疗的广谱抗菌素（Gan等人，2014；Zhao和Wang，2013）。 四环素作为抗生素和生长激素在兽医学上的过度使用能引起食品中抗菌素的残留（Asadollahi- Baboli和Mani - Varnosfaderani 2014；Tan等人，2013b）。 食品中四环素的残留能对人体健康产生不良的毒副作用并使兽体和人体对抗生素产生细菌抗药性（Moreira等人，2010；Tan等人，2013b）。 过敏性反应、牙齿发黄和肝功损害是四环素类药物的毒副作用（Mu等人，2012）。   因此,有选择性的、快速灵敏的四环素检测方法将会受到极大的关注。目前四环素的检测方法有：免疫测定法（Aga等人，2003；Jeon和Rhee Paeng，2008）、高效液相色谱法（HPLC）（Cinquina等人，2003；Shariati等人，2009）、微生物测定法（Idowu等人，2010；Kurittu等人，2000）、化学发光法（Townshend等人，2005）和毛细管电泳法（CE）（Kowalski，2008；Tong等人，2009）。 一般来说，这些方法都需要复杂和昂贵的仪器并需要漫长的周转时间（Chen等人，2014；Tan等人，2013b）。适体是通过名为SELEX（指数富集的配基系统进化技术）的体外方法得到的单链寡核苷酸（Lian等人，2015）。 这些适体对它们的靶标作出反应，从小物质到蛋白质，甚至具有高特异性和亲合力的细胞（Huang等人，2014；Zhao等人，2015）。 适体与抗体相比具有低成本、热稳定性优良、易于生产和改进以及缺乏毒性和免疫原性的优点（Bai等人，2013；Lian等人，2015；Luo等人，2014；Shan等人，2014）。由于这些独特的性质,适体在生物传感器方面的应用已受到相当大的关注（Evtugyn等人，2013；Taghdisi等人，2014）。近来，金纳米粒子(AuNPs)由于其具有诸如化学稳定性、高灵敏度、易于合成以及极高的吸收系数等独特的特性已广泛地应用于比色及荧光猝灭适体传感器的开发（Gopinath等人，2014；Liu等人，2014；Tan等人，2013a；Zhang等人，2013）。比色测定是一种分析应用的常用技术,因为只通过肉眼就能够确定目标识别结果（Gopinath等人，2014）。
   在本研究中，首次基于适体和AuNPs设计了一种用于四环素检测的比色三重螺旋分子开关(THMS)系统。与已知的双螺旋DNA分子开关和基于信号适体的分子信标相比THMS表现出独特的优点，包括高灵敏度和高稳定性以及保持原适体的特异性和亲合力（Zheng等人，2011）。 THMS系统通常由一个具有两个臂段的无标记靶标特异性适体序列和一个作为一种信号转导探针(STP)的双标记寡核苷酸组成( Verdian - Doghaei等人，2014; Zheng等人2011）,但是在本研究中使用无标记STP是经济的并且不需要任何特殊的仪器。 在本方案中使用一种截短的8-mer长度的单链DNA适体作为靶向剂,它选择性地与不同的四环素类药物结合( Kwon等人，2014）。
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贝壳英
8楼2016-12-18 13:04:35
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贝壳英

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引用回帖:
8楼: Originally posted by jiangguofeng at 2016-12-18 13:04:35
1.引言
四环素是一种人用和兽用治疗的广谱抗菌素（Gan等人，2014；Zhao和Wang，2013）。 四环素作为抗生素和生长激素在兽医学上的过度使用能引起食品中抗菌素的残留（Asadollahi- Baboli和Mani - Varnosfaderani  ...

谢谢。

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