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贝壳英银虫 (正式写手)
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把下面的英文摘要翻译成中文
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A novel colorimetric triple-helix molecular switch aptasensor for ul- trasensitive detection of tetracycline a b s t r a c t Detection methods of antibiotic residues in blood serum and animal derived foods are of great interest. In this study a colorimetric aptasensor was designed for sensitive, selective and fast detection of tetracycline based on triple-helix molecular switch(THMS) and gold nanoparticles (AuNPs). As a biosensor, THMS shows distinct advantages including high stability, sensitivity and preserving the selectivity and affinity of the original aptamer. In the absence of tetracycline, THMS is stable, leading to the aggregation of AuNPs by salt and anobvious color change from red to blue. In the presence of tetracycline, aptamer binds toitstarget, signal transduction probe(STP) leaves the THMS and adsorbs on the surface of AuNPs. So the well-dispersed AuNPs remain stable against salt-induced aggregation with a red color. The presented aptasensor showed high selectivity toward tetracyclines with a limit of detection as low as 266 pM for tetracycline. The designed aptasensor was successfully applied to detect tetracycline in serum and milk. |
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贝壳英
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1.Introduction Tetracyclines are broad-spectrum antibiotics which are used in human and veterinary therapy (Gan et al. 2014; Zhao and Wang 2013). Excessive use of tetracyclines in veterinary medicine as antibiotics and growth promotors could lead to antibiotic residues in food products(Asadollahi-Baboli and Mani-Varnosfaderani 2014; Tan et al.2013b). Residues of tetracyclines in food products could result in undesirable side effects on human health and emergence of bacterial resistance to antibiotics in both veterinary and human(Moreiraet al.2010; Tan et al.2013b). Allergic reactions, yellowing of teeth and liver damage are among side effects of tetracyclines(Mu et al.2012). Therefore,selective,fast and sensitive methods for detectionof tetracyclines are of great interest.Current methods for detection of tetracyclines are: immunoassays(Aga et al.2003; Jeon and Rhee Paeng2008), high performance liquid chromatography(HPLC) (Cinquina et al.2003; Shariati et al.2009), microbiological methods (Idowu et al.2010; Kurittu et al.2000), chemiluminescence (Townshend et al.2005) and capillary electrophoresis(CE)(Kowalski 2008; Tong et al.2009). Generally these approaches require sophisticated and expensive instruments and need lengthy turnaround time(Chen et al.2014; Tan et al.2013b). Aptamers are single-stranded oligonucleotides,obtained by an in vitro process named SELEX(systematic evolution of ligands by exponential enrichment)(Lian et al.2015). They react to their targets ranging from small substances to proteins and even cells with high specificity and affinity (Huang et al.2014; Zhao et al. 2015). Aptamers possess advantages over antibodies like low cost, excellent thermal stability,ease of production and modification, and lack of toxicity and immunogenicity(Bai et al.2013; Lian et al. 2015; Luo et al.2014; Shan et al.2014). Owing to these unique properties, aptamers have attracted substantial attentions for application in biosensors(Evtugyn et al.2013; Taghdisi et al.2014). Recently gold nanoparticles(AuNPs)have been broadly used in the development of colorimetric and fluorescence quenching aptasensors, because of their unique characteristics such as chemical stability,high sensitivity,ease of synthesis and extremely high absorption coefficient (Gopinath et al.2014; Liu et al.2014; Tan et al. 2013a; Zhang et al. 2013). Colorimetric assay is a common technique for analytical applications, since the target recognition event can be determined only by the naked eye (Gopinath et al. 2014). In this study a colorimetric triple-helix molecular switch (THMS) system was designed for the first time for detection of tetracyclines based on aptamer and AuNPs.THMS presents unique advantages over known double-helix DNA molecular switches and molecular beacon-based signaling aptamers including high sensitivity and stability and preserving the specificity and affinity of the original aptamer (Zheng et al.2011). THMS system usually consists of a label-free target specific aptamer sequence with two arm segments and a dual-labeled oligonucleotide as a signal transduction probe(STP)(Verdian-Doghaei et al.2014; Zheng et al. 2011), but in this study a label-free STP was used which is economical and does not need any special instrument.In this project a truncated 8-mer ssDNA aptamer,which selectively binds to different tetracyclines(Kwon et al.2014), was used as targeting agent. |
7楼2016-12-18 08:23:44
jiangguofeng
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【答案】应助回帖
★ ★ ★ ★ ★ ★ ★ ★ ★ ★
贝壳英(fjtony163代发): 金币+10, 代发 2017-01-19 15:31:44
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贝壳英(fjtony163代发): 金币+10, 代发 2017-01-19 15:31:44
fjtony163: 翻译EPI+1, 代发 2017-01-19 15:31:52
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一种新型的用于四环素超灵敏检测的比色三重螺旋分子开关适体传感器 摘要 血清和动物来源食品中抗菌素残留物的检测方法将会受到极大的关注。于这项研究中，基于三重螺旋分子开关(THMS)和金纳米粒子(AuNPs)设计了一种用于灵敏快速、有选择性的四环素检测方法的比色适体传感器。作为一种生物传感器, THMS显示出明显的优点，包括高稳定性和高灵敏度并能保持原适体的选择性和亲合力。在不存在四环素的情况下, THMS是稳定的,通过盐引起AuNPs聚集和一个由红到蓝明显的变色。 在有四环素存在下,适体结合其靶标,信号转导探针( STP)离开THMS并吸附于AuNPs的表面上。因此充分分散的AuNPs对于盐诱导的聚集保持稳定并带有一种红颜色。提出的这种适体传感器显示出对四环素的高选择性，四环素的检测极限低至266 pM。设计的这种适体传感器成功地应用到了血清和牛奶中四环素的检测当中。 |
2楼2016-12-17 12:56:54
是非对错
铁杆木虫 (正式写手)
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| Abstract: In this paper, 2-chloro-quinoline and phenylboronic acid were used as starting materials, and the main ligand 2-phenylquinoline (pq) was obtained by the reaction of tetrakis (triphenylphosphine) palladium as a catalyst. Then pq was reacted with iridium trichloride (Pq) 2Ir (μ-Cl2) Ir (pq) 2 was synthesized by the reaction of 2-amino-2-methyl-2-propanol with ethylene glycol monoethylether and then acetylation reaction was carried out under alkaline conditions. 2-phenylquinoline-C2, N ') (acetylacetonato) iridium (III) Ir (pq) 2 (acac). The structures of the compounds were characterized by elemental analysis, IR, 1H NMR, 13C NMR, mass spectroscopy and single crystal X-ray diffraction. Their photophysical properties were characterized by UV-Vis absorption spectra and photoluminescence spectra. test. The results show that Ir (pq) 2 (acac) is an electrically neutral octahedral complex with an average Ir-O bond length of 0.21741 (18) nm and an Ir-C bond length of 0.1983 (3) The average length of the N bond is 0.2079 (2) nm, and the strong red emission occurs at 600nm. The synthetic yield is more than 95%. This method is suitable for small batch preparation of Ir (pq) 2 (acac). |
3楼2016-12-17 16:22:38
是非对错
铁杆木虫 (正式写手)
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4楼2016-12-17 16:23:25