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角落里----木虫 (小有名气)
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| Proteomics analysis for enhanced lipid accumulation in oleaginous Chlorella vulgaris under a heterotrophic-Na+ induction two-step regime |
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baiyuefei
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风雪
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角落里----: 金币+5, ★★★★★最佳答案 2016-09-26 16:24:17
sunshan4379: LS-EPI+1, 感谢应助！ 2016-09-26 18:12:00
感谢参与，应助指数 +1
角落里----: 金币+5, ★★★★★最佳答案 2016-09-26 16:24:17
sunshan4379: LS-EPI+1, 感谢应助！ 2016-09-26 18:12:00
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Proteomics analysis for enhanced lipid accumulation in oleaginous Chlorella vulgaris under a heterotrophic-Na+ induction two-step regime 作者:Li, YQ (Li, Yuqin)[ 1 ] ; Mu, JX (Mu, Jinxiu)[ 1 ] ; Chen, D (Chen, Di)[ 1 ] ; Xu, H (Xu, Hua)[ 1 ] ; Han, FX (Han, Fangxin)[ 1 ] ; Feng, B (Feng, Bo)[ 1 ] ; Zeng, HY (Zeng, Hongyan)[ 1 ] BIOTECHNOLOGY LETTERS 卷: 37 期: 5 页: 1021-1030 DOI: 10.1007/s10529-014-1758-0 出版年: MAY 2015 查看期刊信息 BIOTECHNOLOGY LETTERS 出版商 SPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS ISSN: 0141-5492 eISSN: 1573-6776 研究领域 Biotechnology & Applied Microbiology 摘要 A heterotrophic-Na+ induction (HNI) two-step regime was developed to enhance lipid accumulation in oleaginous Chlorella vulgaris. C. vulgaris was cultivated heterotrophically to a biomass of 7.8 g l(-1) in 120 h. The cells were re-suspended in fresh media supplemented with 0.5 M NaCl followed by 12 h growth to accumulate lipid to 53.4 % (w/w). The lipid productivity (625 mg l(-1) day(-1)) achieved with HNI was better than that using heterotrophy alone (405 mg l(-1) day(-1)). To promote possible applications of HNI strategy in other microalgal species, the lipid triggers and potential molecular pathways associated with lipid biosynthesis were investigated. Malic enzyme and acyl-CoA-binding protein were key metabolic checkpoints found to modulate lipid biosynthesis in cells. These results provide the foundation to develop high-lipid engineering miroalgae for industrialization of biodiesel. 关键词 作者关键词:Bioinformatics; Biomass; Chlorella vulgaris; Heterotrophic-Na+ induction; Lipid; Proteomics KeyWords Plus:NACL STRESS; SALT; TRIACYLGLYCEROLS; PROTEINS; NITROGEN; ROOTS 作者信息 通讯作者地址: Li, YQ (通讯作者) Xiangtan Univ, Sch Chem Engn, Xiangtan 411105, Peoples R China. 增强组织信息的名称 Xiangtan University 地址: [ 1 ] Xiangtan Univ, Sch Chem Engn, Xiangtan 411105, Peoples R China 增强组织信息的名称 Xiangtan University 电子邮件地址:yuqinli2004@126.com 基金资助致谢 基金资助机构 授权号 National Natural Science Foundation of China 21306161 China Postdoctoral Science Foundation 2012M521531 2014T70775 Hunan Provincial Natural Science Foundation of China 13JJ3062 查看基金资助信息关闭基金资助信息 This work was supported by National Natural Science Foundation of China (21306161), China Postdoctoral Science Foundation Funded Project (2012M521531 and 2014T70775), and Hunan Provincial Natural Science Foundation of China (13JJ3062). 出版商 SPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS 类别 / 分类 研究方向:Biotechnology & Applied Microbiology Web of Science 类别:Biotechnology & Applied Microbiology 文献信息 文献类型:Article 语种:English 入藏号: WOS:000352641700008 PubMed ID: 25548117 ISSN: 0141-5492 eISSN: 1573-6776 其他信息 IDS 号: CF6BP Web of Science 核心合集中的 "引用的参考文献": 20 Web of Science 核心合集中的 "被引频次": 0 JCR® 类别 JCR 分区 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Q3 数据来自第 2015 版 Journal Citation Reports® |
2楼2016-09-26 16:05:28