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| Proteomics analysis for enhanced lipid accumulation in oleaginous Chlorella vulgaris under a heterotrophic-Na+ induction two-step regime |
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½ÇÂäÀï----: ½ð±Ò+5, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸ 2016-09-26 16:24:17
sunshan4379: LS-EPI+1, ¸ÐлӦÖú£¡ 2016-09-26 18:12:00
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½ÇÂäÀï----: ½ð±Ò+5, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸ 2016-09-26 16:24:17
sunshan4379: LS-EPI+1, ¸ÐлӦÖú£¡ 2016-09-26 18:12:00
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Proteomics analysis for enhanced lipid accumulation in oleaginous Chlorella vulgaris under a heterotrophic-Na+ induction two-step regime ×÷Õß:Li, YQ (Li, Yuqin)[ 1 ] ; Mu, JX (Mu, Jinxiu)[ 1 ] ; Chen, D (Chen, Di)[ 1 ] ; Xu, H (Xu, Hua)[ 1 ] ; Han, FX (Han, Fangxin)[ 1 ] ; Feng, B (Feng, Bo)[ 1 ] ; Zeng, HY (Zeng, Hongyan)[ 1 ] BIOTECHNOLOGY LETTERS ¾í: 37 ÆÚ: 5 Ò³: 1021-1030 DOI: 10.1007/s10529-014-1758-0 ³ö°æÄê: MAY 2015 ²é¿´ÆÚ¿¯ÐÅÏ¢ BIOTECHNOLOGY LETTERS ³ö°æÉÌ SPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS ISSN: 0141-5492 eISSN: 1573-6776 Ñо¿ÁìÓò Biotechnology & Applied Microbiology ÕªÒª A heterotrophic-Na+ induction (HNI) two-step regime was developed to enhance lipid accumulation in oleaginous Chlorella vulgaris. C. vulgaris was cultivated heterotrophically to a biomass of 7.8 g l(-1) in 120 h. The cells were re-suspended in fresh media supplemented with 0.5 M NaCl followed by 12 h growth to accumulate lipid to 53.4 % (w/w). The lipid productivity (625 mg l(-1) day(-1)) achieved with HNI was better than that using heterotrophy alone (405 mg l(-1) day(-1)). To promote possible applications of HNI strategy in other microalgal species, the lipid triggers and potential molecular pathways associated with lipid biosynthesis were investigated. Malic enzyme and acyl-CoA-binding protein were key metabolic checkpoints found to modulate lipid biosynthesis in cells. These results provide the foundation to develop high-lipid engineering miroalgae for industrialization of biodiesel. ¹Ø¼ü´Ê ×÷Õ߹ؼü´Ê:Bioinformatics; Biomass; Chlorella vulgaris; Heterotrophic-Na+ induction; Lipid; Proteomics KeyWords Plus:NACL STRESS; SALT; TRIACYLGLYCEROLS; PROTEINS; NITROGEN; ROOTS ×÷ÕßÐÅÏ¢ ͨѶ×÷ÕßµØÖ·: Li, YQ (ͨѶ×÷Õß) Xiangtan Univ, Sch Chem Engn, Xiangtan 411105, Peoples R China. ÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ Xiangtan University µØÖ·: [ 1 ] Xiangtan Univ, Sch Chem Engn, Xiangtan 411105, Peoples R China ÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ Xiangtan University µç×ÓÓʼþµØÖ·:yuqinli2004@126.com »ù½ð×ÊÖúÖÂл »ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ National Natural Science Foundation of China 21306161 China Postdoctoral Science Foundation 2012M521531 2014T70775 Hunan Provincial Natural Science Foundation of China 13JJ3062 ²é¿´»ù½ð×ÊÖúÐÅÏ¢¹Ø±Õ»ù½ð×ÊÖúÐÅÏ¢ This work was supported by National Natural Science Foundation of China (21306161), China Postdoctoral Science Foundation Funded Project (2012M521531 and 2014T70775), and Hunan Provincial Natural Science Foundation of China (13JJ3062). ³ö°æÉÌ SPRINGER, VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS Àà±ð / ·ÖÀà Ñо¿·½Ïò:Biotechnology & Applied Microbiology Web of Science Àà±ð:Biotechnology & Applied Microbiology ÎÄÏ×ÐÅÏ¢ ÎÄÏ×ÀàÐÍ:Article ÓïÖÖ:English Èë²ØºÅ: WOS:000352641700008 PubMed ID: 25548117 ISSN: 0141-5492 eISSN: 1573-6776 ÆäËûÐÅÏ¢ IDS ºÅ: CF6BP Web of Science ºËÐĺϼ¯ÖÐµÄ "ÒýÓõIJο¼ÎÄÏ×": 20 Web of Science ºËÐĺϼ¯ÖÐµÄ "±»ÒýƵ´Î": 0 JCR® Àà±ð JCR ·ÖÇø BIOTECHNOLOGY & APPLIED MICROBIOLOGY Q3 Êý¾ÝÀ´×ÔµÚ 2015 °æ Journal Citation Reports® |
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