| 查看: 675 | 回复: 3 | ||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | ||
soloaaaa木虫 (正式写手)
|
[求助]
PET-HM是个什么质粒啊，求助相关信息 已有1人参与
|
|
|
看文献见到了个PET-HM质粒，查不到相关的信息，求教。 文献原文：The expression plasmid PET-HM was introduced into the T7-RNA-polymerasecontaining host E. coli BL21(DE3) (Studier et al., 1990). T7 RNA polymerase is used to drive the expression of DNA located downstream of the T7 promoter of the PET vector. Cells harboring PET-HM or PET-3a (control) were each grown under agitation at 37°C in Luria-Bertani medium containing 100 pg carbenicillidml, in the presence or absence of 100 pM lipoic acid, to an A,,, of 0.5 (about 2-2.5 h). Induction of the target prcitein was started by addition of isopropylthio-b-D-galactopyranclside to 0.4 mM. To monitor the induction of H protein 1 ml of culture was sampled at various times after induction. The cells were centrifuged (14000Xg, 1 min) and suspended in 100 p1 5DS/ PAGE sample buffer (Laemmli, 1970). After heating at 100°C for 3 min, 10 p1 of the sample (corresponding to 100 pl of culture) were subjected to |
回复此楼» 猜你喜欢
筑牢营养安全线：以精准检测，护健康基石
已经有0人回复
-
推荐一些20种氨基酸检测的实际应用案例
已经有0人回复
-
化学工程及工业化学论文润色/翻译怎么收费?
已经有215人回复
-
不合理蛙科研实验中的趣事：实验器材的 “乌龙”
已经有0人回复
-
不合理蛙科研实验中的趣事：和实验材料的 “斗智斗勇”
已经有0人回复
-
蛋白质检测：精准分析，解锁生物分子的密码
已经有0人回复
-
不合理蛙科研实验之小鼠实验：严谨设计，解析生命机制的重要载体
已经有0人回复
-
不合理蛙科研实验之重金属检测：精准筛查，守护健康与环境的防线
已经有0人回复
-
不合理蛙科研实验之“蛙测重金属我背锅三千”
已经有0人回复
-
不合理蛙科研实验之“鼠逃三次我发三篇SCI”
已经有0人回复
4楼2016-08-09 14:41:10
【答案】应助回帖
★ ★ ★ ★ ★ ★
感谢参与，应助指数 +1
soloaaaa: 金币+5, ★★★★★最佳答案 2016-08-09 14:21:40
xn8008: 金币+1, 欢迎发帖交流 2016-08-10 07:24:40
感谢参与，应助指数 +1
soloaaaa: 金币+5, ★★★★★最佳答案 2016-08-09 14:21:40
xn8008: 金币+1, 欢迎发帖交流 2016-08-10 07:24:40
|
楼主，你再把文献好好看一看PET-HM应该就是PET-3a装了目的基因的表达载体，重新编了个名字。 原文是不是这个：“The Ndel-BamH1 fragment was subcloned into the expression vector PET-3a (Stratagene), digested with Ndel and BamHl , to yield the expression plasmid PET-HM.” |
2楼2016-08-09 13:53:45
soloaaaa
木虫 (正式写手)
- 应助: 48 (小学生)
- 金币: 2411.7
- 散金: 29
- 红花: 1
- 帖子: 381
- 在线: 199.1小时
- 虫号: 820780
- 注册: 2009-08-03
- 性别: GG
- 专业: 绿色有机化学
是我错了，没仔细看~ 谢谢啦~ 你也做这个吗？ 3楼2016-08-09 14:22:45