| ²é¿´: 234 | »Ø¸´: 1 | |||
| µ±Ç°Ö÷ÌâÒѾ´æµµ¡£ | |||
[½»Á÷]
ÇëÎÊBio-rad protein assay ʲôÒâ˼£¿
|
|||
|
ÎÒÊÇѧ·ÖÎöµÄ£¬¶ÔһЩÉúÎïµÄ¶«Î÷²»¹»Á˽⣬¿´ÎÄÏ×ÖÐÓС°Bio-rad protein assay(Bio-rad Laboratories, Hercules, CA)¡±£¬ÊÇʲôÒâ˼£¿Ó¦¸ÃÊÇÒ»ÖÖ¶¨Á¿²â¶¨µ°°×º¬Á¿µÄ·½·¨°É£¿ Õû¸ö¾ä×ÓÊÇÕâÑùµÄ¡°the protein content of the combined supernatant was measured using a Bio-rad protein assay (Bio-rad Laboratories, Hercules, CA) with bovine serum albumin as a protein standard.¡± |
» ²ÂÄãϲ»¶
291Çóµ÷¼Á
ÒѾÓÐ12È˻ظ´
317Çóµ÷¼Á
ÒѾÓÐ5È˻ظ´
0703»¯Ñ§Çóµ÷¼Á£¬¸÷λÀÏʦ¿´¿´ÎÒ£¡£¡£¡
ÒѾÓÐ3È˻ظ´
339Çóµ÷¼Á
ÒѾÓÐ5È˻ظ´
287Çóµ÷¼Á
ÒѾÓÐ8È˻ظ´
Ò»Ö¾Ô¸±±¾©»¯¹¤´óѧ 070300 ѧ˶ 336·Ö Çóµ÷¼Á
ÒѾÓÐ8È˻ظ´
085602 »¯¹¤×¨Ë¶ 338·Ö Çóµ÷¼Á
ÒѾÓÐ5È˻ظ´
0856£¬²ÄÁÏÓ뻯¹¤321·ÖÇóµ÷¼Á
ÒѾÓÐ4È˻ظ´
Ò»Ö¾Ô¸±±¾©»¯¹¤´óѧ²ÄÁÏÓ뻯¹¤ 264·Ö¸÷¿Æ¹ýAÇø¹ú¼ÒÏß
ÒѾÓÐ10È˻ظ´
266Çóµ÷¼Á
ÒѾÓÐ8È˻ظ´
liujiahui
ľ³æ (ÖøÃûдÊÖ)
- Ó¦Öú: 1 (Ó×¶ùÔ°)
- ¹ó±ö: 0.035
- ½ð±Ò: 2656.5
- É¢½ð: 3811
- ºì»¨: 9
- Ìû×Ó: 1059
- ÔÚÏß: 294.7Сʱ
- ³æºÅ: 598151
- ×¢²á: 2008-09-10
- רҵ: ÎÞ»úÄÉÃ×»¯Ñ§
|
Bio-Rad Protein Assay µã»÷´ÎÊý£º30 ·¢±íÓÚ£º2008-07-21 17:59¡¡×ªÔØÇë×¢Ã÷À´×Ô¶¡ÏãÔ° À´Ô´£º»¥ÁªÍø Introduction The Bio-Rad Protein Assay, based on the method of Bradford, is a simple and accurate procedure for determining concentration of solubilized protein. It involves the addition of an acidic dye to protein solution,and subsequent measurement at 595 nm with a spectrophotometer or microplate reader. Comparison to a standard curve provides a relative measurement of protein concentration. ¿ÉÄÜÓÐЩÂÒ£¬Áí£º The absorbance maximum for an acidic solution of Coomassie® Brilliant Blue G-250 dye shifts from 465 nm to 595 nm when binding to protein occurs ²Î¿¼×ÊÁÏ£º http://www.namipan.com/d/biorad% ... 52b09222859da3b0100 Áí£º ¡ù À´Ô´:¡¤BBS ˮľÇ廪վ smth.edu.cn¡¤[FROM: 166.111.30.107] ·¢ÐÅÈË: aptamer (°¢ÍÁ£Å¬Á¦ÊµÑéÖÐ), ÐÅÇø: LifeScience ±ê Ìâ: Re: Bio-Rad protein assay ÊÇʲô¶«¶«°¡£¿ ·¢ÐÅÕ¾: BBS ˮľÇ廪վ (Wed Oct 9 15:57:38 2002), Õ¾ÄÚÐżþ Bio-RadÊÇÒ»¼ÒÉúÎ﹫˾µÄÃû×Ö ÕâÀïµÄBio-Rad protein assay dyeÓ¦¸ÃÊÇÒ»ÖÖBio-RadµÄÓÃÒÔµ°°×Öʶ¨Á¿µÄȾɫÊÔ¼Á [ Last edited by liujiahui on 2008-11-12 at 19:00 ] |
2Â¥2008-11-12 18:58:50














»Ø¸´´ËÂ¥
20