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lliumei912

[交流] 请问Bio-rad protein assay 什么意思?

我是学分析的,对一些生物的东西不够了解,看文献中有“Bio-rad protein assay(Bio-rad Laboratories, Hercules, CA)”,是什么意思?应该是一种定量测定蛋白含量的方法吧?
整个句子是这样的“the protein content of the combined supernatant was measured using a Bio-rad protein assay (Bio-rad Laboratories, Hercules, CA) with bovine serum albumin as a protein standard.”
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liujiahui

木虫 (著名写手)

Bio-Rad Protein Assay
点击次数:30  发表于:2008-07-21 17:59 转载请注明来自丁香园
来源:互联网

Introduction

The Bio-Rad Protein Assay, based on the method of Bradford, is a simple and accurate procedure for determining concentration of solubilized protein. It involves the addition of an acidic dye to protein solution,and subsequent measurement at 595 nm with a spectrophotometer or microplate reader. Comparison to a standard curve provides a relative measurement of protein concentration.

可能有些乱,另:

The absorbance maximum for an acidic solution of
Coomassie® Brilliant Blue G-250 dye shifts from 465 nm to 595 nm when
binding to protein occurs

参考资料:
http://www.namipan.com/d/biorad% ... 52b09222859da3b0100

另:
※ 来源:·BBS 水木清华站 smth.edu.cn·[FROM: 166.111.30.107]
发信人: aptamer (阿土-努力实验中), 信区: LifeScience      
标  题: Re: Bio-Rad protein assay  是什么东东啊?
发信站: BBS 水木清华站 (Wed Oct  9 15:57:38 2002), 站内信件

Bio-Rad是一家生物公司的名字
这里的Bio-Rad protein assay dye应该是一种Bio-Rad的用以蛋白质定量的染色试剂

[ Last edited by liujiahui on 2008-11-12 at 19:00 ]
2楼2008-11-12 18:58:50
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