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G欧尼~

新虫 (小有名气)

[求助] 求翻译一篇关于生物正交化学英文文献中的几句话,谢谢!已有1人参与

Furthermore, quadricyclane was stable in the presence of bovine serum albumin (BSA) and cell culture media under conditions emulating those necessary for metabolic labeling experiments.
Complex 14 has a large NIR absorption band at 850 nmthat is not present in the adduct with quadricyclane, allowing for pseudo-first-order kinetic measurements by absorption spectroscopy.
We were excited to see that 15 selectively labeled QC-BSA in a time-dependent manner with very little background labeling of unmodified BSA, even upon prolonged exposure of the Western blot
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xn8008

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G欧尼~: 金币+10, ★★★★★最佳答案 2016-05-28 21:10:21
此外,四环庚烷可以作为模拟细胞代谢实验标记物,在培养所用的载体牛血清白蛋白(BSA)中稳定的存在。
在假一级酶催化反应的红外波谱测定中可以发现,复合物在红外线850nm处有强大的吸收峰,但并不与四环庚烷同时出现在吸收波谱中。
我们很高兴的发现选择性标记的QC-BSA在时间依赖上与未作修改的BSA相比,背景更小,这种背景干扰甚至在western blot实验的长期暴露中仍然保持良好。
奋斗是我们前进的动力!
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