| 查看: 896 | 回复: 5 | |||
| 当前只显示满足指定条件的回帖,点击这里查看本话题的所有回帖 | |||
zhangjingwjs新虫 (小有名气)
|
[交流]
问题 已有2人参与
|
||
|
Nowadays, I used the diluted spreading method to evaluate the density\population of the soil. Firstly, 0.5 g of fresh soil was collected and put in a triangular flask with some glass beads. Then shaking for 15 min, subsequently cooling down about 2 min, next smoking 10 ml into the 15 ml tubes, which was treated as 10-dilution solution. then i ml from the 10-1 was taken and transferred to another 9 ml sterile water, which was as 10-2. In a similar way, I got 10-3, 10-4, 10-5, 10-6, 10-7, 10-8, 10-9. Finally, 1 ml of each diluted solution was spreading on the R2A plates, then put in the stove at 37 degree temperature for two days. three replicates of each treatment. Now, my question is that why I found there was nothing on some plates but not all, for example one of the 10-6 and 10-5. while on the 10-9 plates, there were much bacteria colonies, which was so strange. Now i am wondering What happened during the operation <> who knows ? please share your experiences here, thanks in advance! [ Last edited by zhangjingwjs on 2016-6-20 at 00:39 ] |
» 本帖已获得的红花(最新10朵)
» 猜你喜欢
290调剂生物0860
已经有32人回复
271求调剂
已经有28人回复
105500药学求调剂
已经有4人回复
274求调剂
已经有10人回复
335求调剂
已经有20人回复
化工学硕294分,求导师收留
已经有33人回复
290求调剂
已经有22人回复
291 求调剂
已经有36人回复
食品与营养(0955)271求调剂
已经有16人回复
22408 312求调剂
已经有10人回复
zhangjingwjs
新虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 7.8
- 散金: 240
- 红花: 6
- 帖子: 123
- 在线: 49.1小时
- 虫号: 2058795
- 注册: 2012-10-13
- 专业: 文化社会学
5楼2016-05-30 16:10:40
yangli_sisi
铜虫 (小有名气)
- 应助: 22 (小学生)
- 金币: 123.2
- 红花: 1
- 帖子: 93
- 在线: 45.1小时
- 虫号: 3376798
- 注册: 2014-08-21
- 专业: 色谱分析
★
小木虫: 金币+0.5, 给个红包,谢谢回帖
小木虫: 金币+0.5, 给个红包,谢谢回帖
|
maby your dilution is not enough, the density of each planet is not proportional 发自小木虫Android客户端 |
2楼2016-05-27 18:46:13
yangli_sisi
铜虫 (小有名气)
- 应助: 22 (小学生)
- 金币: 123.2
- 红花: 1
- 帖子: 93
- 在线: 45.1小时
- 虫号: 3376798
- 注册: 2014-08-21
- 专业: 色谱分析
3楼2016-05-27 18:51:31
4楼2016-05-28 21:28:07














回复此楼
白若薇

10