| 查看: 895 | 回复: 5 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
zhangjingwjs新虫 (小有名气)
|
[交流]
问题 已有2人参与
|
||
|
Nowadays, I used the diluted spreading method to evaluate the density\population of the soil. Firstly, 0.5 g of fresh soil was collected and put in a triangular flask with some glass beads. Then shaking for 15 min, subsequently cooling down about 2 min, next smoking 10 ml into the 15 ml tubes, which was treated as 10-dilution solution. then i ml from the 10-1 was taken and transferred to another 9 ml sterile water, which was as 10-2. In a similar way, I got 10-3, 10-4, 10-5, 10-6, 10-7, 10-8, 10-9. Finally, 1 ml of each diluted solution was spreading on the R2A plates, then put in the stove at 37 degree temperature for two days. three replicates of each treatment. Now, my question is that why I found there was nothing on some plates but not all, for example one of the 10-6 and 10-5. while on the 10-9 plates, there were much bacteria colonies, which was so strange. Now i am wondering What happened during the operation <> who knows ? please share your experiences here, thanks in advance! [ Last edited by zhangjingwjs on 2016-6-20 at 00:39 ] |
回复此楼» 本帖已获得的红花（最新10朵）
白若薇» 猜你喜欢
RY：中国产出的科学垃圾论文，绝对数量和比例都世界第一
已经有16人回复
-
297，工科调剂?河南农业大学本科
已经有7人回复
-
药学求调剂
已经有5人回复
-
食品与营养（0955）271求调剂
已经有14人回复
-
366求调剂
已经有10人回复
-
085408光电信息工程专硕355一志愿长春光机所调剂
已经有13人回复
-
一志愿鲁东大学071000生物学学硕初试分数276求调剂
已经有25人回复
-
药学305求调剂
已经有9人回复
-
转长聘了
已经有6人回复
-
22408 312求调剂
已经有9人回复
zhangjingwjs
新虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 7.8
- 散金: 240
- 红花: 6
- 帖子: 123
- 在线: 49.1小时
- 虫号: 2058795
- 注册: 2012-10-13
- 专业: 文化社会学
5楼2016-05-30 16:10:40
yangli_sisi
铜虫 (小有名气)
- 应助: 22 (小学生)
- 金币: 123.2
- 红花: 1
- 帖子: 93
- 在线: 45.1小时
- 虫号: 3376798
- 注册: 2014-08-21
- 专业: 色谱分析
★
小木虫: 金币+0.5, 给个红包，谢谢回帖
小木虫: 金币+0.5, 给个红包，谢谢回帖
|
maby your dilution is not enough, the density of each planet is not proportional 发自小木虫Android客户端 |
2楼2016-05-27 18:46:13
yangli_sisi
铜虫 (小有名气)
- 应助: 22 (小学生)
- 金币: 123.2
- 红花: 1
- 帖子: 93
- 在线: 45.1小时
- 虫号: 3376798
- 注册: 2014-08-21
- 专业: 色谱分析
3楼2016-05-27 18:51:31
4楼2016-05-28 21:28:07
