24小时热门版块排行榜    

查看: 1007  |  回复: 5

zhangjingwjs

新虫 (小有名气)

[交流] 问题 已有2人参与

Nowadays, I used the diluted spreading method to evaluate the density\population of the soil.
Firstly, 0.5 g of fresh soil was collected and put in a triangular flask with some glass beads.
Then shaking for 15 min, subsequently cooling down about 2 min, next smoking 10 ml into the 15 ml tubes, which was treated as 10-dilution solution.
then i ml from the 10-1 was taken and transferred to another 9 ml sterile water, which was as 10-2.
In a similar way, I got 10-3, 10-4, 10-5, 10-6, 10-7, 10-8, 10-9.
Finally, 1 ml of each diluted solution was spreading on the R2A plates, then put in the stove at 37 degree temperature for two days. three replicates of each treatment.
Now, my question is that why I found there was nothing on some plates but not all, for example one of the 10-6 and 10-5.
while on the 10-9 plates, there were much bacteria colonies, which was so strange.
Now i am wondering What happened during the operation <>
who knows ? please share your experiences here, thanks in advance!

[ Last edited by zhangjingwjs on 2016-6-20 at 00:39 ]
回复此楼
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

yangli_sisi

铜虫 (小有名气)


小木虫: 金币+0.5, 给个红包,谢谢回帖
maby your dilution is not enough, the density of each planet is not proportional

发自小木虫Android客户端
2楼2016-05-27 18:46:13
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

yangli_sisi

铜虫 (小有名气)


小木虫: 金币+0.5, 给个红包,谢谢回帖
when you make a new solution ,you should shake for a while

发自小木虫Android客户端
3楼2016-05-27 18:51:31
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
送红花一朵
4楼2016-05-28 21:28:07
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

zhangjingwjs

新虫 (小有名气)

引用回帖:
3楼: Originally posted by yangli_sisi at 2016-05-27 18:51:31
when you make a new solution ,you should shake for a while

I did a second time, of course each dilution three replicates, but the new problem is in the same dilution, some of the plates can grow bacteria , but some can not, what's the reason >? thanks YangLi
5楼2016-05-30 16:10:40
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

yangli_sisi

铜虫 (小有名气)


小木虫: 金币+0.5, 给个红包,谢谢回帖
引用回帖:
5楼: Originally posted by zhangjingwjs at 2016-05-30 16:10:40
I did a second time, of course each dilution three replicates, but the new problem is in the same dilution, some of the plates can grow bacteria , but some can not, what's the reason >? thanks Ya ...

It happens to me too. Just do it again. I think it may be caused by over dilution. By the way, I translate 0.1ml of dilution to each plate, and then evenly coated, maybe you can grow bacteria. Do you sure your medium and the activity of your bacteria are all right?
6楼2016-05-31 14:48:12
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
相关版块跳转 我要订阅楼主 zhangjingwjs 的主题更新
普通表情 高级回复 (可上传附件)
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[基金申请] Filecode 又变了,巨变 +3 WH3796 2026-08-12 4/200 2026-08-13 14:13 by 小木虫6752397
[基金申请] 不应该看fileCode +6 且听虎啸 2026-08-12 8/400 2026-08-13 12:07 by 小木虫6752397
[基金申请] FileCode能看出啥? +8 要乐观耀哥 2026-08-10 26/1300 2026-08-13 12:01 by Tide man
[基金申请] 关于Filecode分析方法 +9 majunge000 2026-08-10 11/550 2026-08-13 10:40 by ZH远山
[基金申请] 重要来源:本周末出结果 +9 瞬息宇宙 2026-08-12 9/450 2026-08-13 10:12 by Vivilian
[基金申请] 我的国基提前知道中了,可是同事的操作让我实在接受不了,怎么会有这样的人 +9 家与远方 2026-08-10 14/700 2026-08-13 09:41 by 臭臭不臭01
[基金申请] 结合人工智能,周易传统文化,filecode打分制来了,3分以上希望很大。 +3 Tide man 2026-08-12 4/200 2026-08-13 08:35 by ZJTJZ
[基金申请] 2019年青年基金涵评意见,大家看看几个A,几个B? +11 Tide man 2026-08-11 11/550 2026-08-13 07:35 by 撸猫猫
[基金申请] 应该是93bebmhtak前后十一个字符比较关键 +22 Lanmanbaby 2026-08-09 36/1800 2026-08-12 22:50 by sdfapple719
[基金申请] filecode +13 documentary 2026-08-10 15/750 2026-08-12 15:56 by 云上清扬
[基金申请] 综述论文作为代表作会不会影响评审专家的印象分? +11 yufeiwaner 2026-08-09 13/650 2026-08-12 08:17 by yufeiwaner
[基金申请] 是这周出结果还是下周出结果? +3 yuleib84 2026-08-11 3/150 2026-08-11 21:53 by jnhyjjm
[基金申请] 有时候,自然基金真的不能太认真 (我的申报经验) +3 majunge000 2026-08-11 4/200 2026-08-11 20:13 by lch2012
[基金申请] 帮忙看看fileCode +7 wwncly 2026-08-10 13/650 2026-08-11 19:36 by 冰心玉壶晴
[硕博家园] 读博的好处 +3 lnee 2026-08-11 3/150 2026-08-11 18:10 by 希望我好好的
[基金申请] 基金中了 +15 laoda193707 2026-08-06 15/750 2026-08-11 00:11 by jiafei2190
[基金申请] 确定了,国自然21号放榜 +6 布布和一二 2026-08-10 7/350 2026-08-10 19:15 by 2000zf36392
[基金申请] 2026国自然放榜时间 +9 布布和一二 2026-08-08 9/450 2026-08-10 11:22 by xxxx2020
[基金申请] 化学口download_prp&amp;fileCode的固定段好像这几天一直没变,有变的大神么? +3 Tide man 2026-08-07 4/200 2026-08-07 22:39 by Tide man
[基金申请] 固定端突然变了,今天 +6 archvillain 2026-08-06 10/500 2026-08-07 16:03 by 医学老男孩
信息提示
请填处理意见