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zhangjingwjs

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Nowadays, I used the diluted spreading method to evaluate the density\population of the soil.
Firstly, 0.5 g of fresh soil was collected and put in a triangular flask with some glass beads.
Then shaking for 15 min, subsequently cooling down about 2 min, next smoking 10 ml into the 15 ml tubes, which was treated as 10-dilution solution.
then i ml from the 10-1 was taken and transferred to another 9 ml sterile water, which was as 10-2.
In a similar way, I got 10-3, 10-4, 10-5, 10-6, 10-7, 10-8, 10-9.
Finally, 1 ml of each diluted solution was spreading on the R2A plates, then put in the stove at 37 degree temperature for two days. three replicates of each treatment.
Now, my question is that why I found there was nothing on some plates but not all, for example one of the 10-6 and 10-5.
while on the 10-9 plates, there were much bacteria colonies, which was so strange.
Now i am wondering What happened during the operation <>
who knows ? please share your experiences here, thanks in advance!

[ Last edited by zhangjingwjs on 2016-6-20 at 00:39 ]
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1楼 2016-05-27 17:34:24
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yangli_sisi

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maby your dilution is not enough, the density of each planet is not proportional

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2楼2016-05-27 18:46:13
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yangli_sisi

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when you make a new solution ,you should shake for a while

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3楼2016-05-27 18:51:31
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4楼2016-05-28 21:28:07
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zhangjingwjs

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3楼: Originally posted by yangli_sisi at 2016-05-27 18:51:31
when you make a new solution ,you should shake for a while

I did a second time, of course each dilution three replicates, but the new problem is in the same dilution, some of the plates can grow bacteria , but some can not, what's the reason >? thanks YangLi
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5楼2016-05-30 16:10:40
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yangli_sisi

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5楼: Originally posted by zhangjingwjs at 2016-05-30 16:10:40
I did a second time, of course each dilution three replicates, but the new problem is in the same dilution, some of the plates can grow bacteria , but some can not, what's the reason >? thanks Ya ...

It happens to me too. Just do it again. I think it may be caused by over dilution. By the way, I translate 0.1ml of dilution to each plate, and then evenly coated, maybe you can grow bacteria. Do you sure your medium and the activity of your bacteria are all right?
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6楼2016-05-31 14:48:12
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