| 查看: 1052 | 回复: 5 | |||
zhangjingwjs新虫 (小有名气)
|
[交流]
问题 已有2人参与
|
|
Nowadays, I used the diluted spreading method to evaluate the density\population of the soil. Firstly, 0.5 g of fresh soil was collected and put in a triangular flask with some glass beads. Then shaking for 15 min, subsequently cooling down about 2 min, next smoking 10 ml into the 15 ml tubes, which was treated as 10-dilution solution. then i ml from the 10-1 was taken and transferred to another 9 ml sterile water, which was as 10-2. In a similar way, I got 10-3, 10-4, 10-5, 10-6, 10-7, 10-8, 10-9. Finally, 1 ml of each diluted solution was spreading on the R2A plates, then put in the stove at 37 degree temperature for two days. three replicates of each treatment. Now, my question is that why I found there was nothing on some plates but not all, for example one of the 10-6 and 10-5. while on the 10-9 plates, there were much bacteria colonies, which was so strange. Now i am wondering What happened during the operation <> who knows ? please share your experiences here, thanks in advance! [ Last edited by zhangjingwjs on 2016-6-20 at 00:39 ] |
回复此楼» 本帖已获得的红花（最新10朵）
白若薇» 猜你喜欢
我应该选哪条合成路线让实验成功率高一些？
已经有8人回复
-
烦躁
已经有8人回复
-
入职后第一次管课题组的专利缴费，对账才发现官费比别人多掏了一倍
已经有3人回复
-
各位大佬，路线设计求助
已经有7人回复
yangli_sisi
铜虫 (小有名气)
- 应助: 22 (小学生)
- 金币: 123.2
- 红花: 1
- 帖子: 93
- 在线: 45.1小时
- 虫号: 3376798
- 注册: 2014-08-21
- 专业: 色谱分析
★
小木虫: 金币+0.5, 给个红包，谢谢回帖
小木虫: 金币+0.5, 给个红包，谢谢回帖
|
maby your dilution is not enough, the density of each planet is not proportional 发自小木虫Android客户端 |
2楼2016-05-27 18:46:13
yangli_sisi
铜虫 (小有名气)
- 应助: 22 (小学生)
- 金币: 123.2
- 红花: 1
- 帖子: 93
- 在线: 45.1小时
- 虫号: 3376798
- 注册: 2014-08-21
- 专业: 色谱分析
3楼2016-05-27 18:51:31
4楼2016-05-28 21:28:07
zhangjingwjs
新虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 7.8
- 散金: 240
- 红花: 6
- 帖子: 123
- 在线: 49.1小时
- 虫号: 2058795
- 注册: 2012-10-13
- 专业: 文化社会学
5楼2016-05-30 16:10:40
yangli_sisi
铜虫 (小有名气)
- 应助: 22 (小学生)
- 金币: 123.2
- 红花: 1
- 帖子: 93
- 在线: 45.1小时
- 虫号: 3376798
- 注册: 2014-08-21
- 专业: 色谱分析
6楼2016-05-31 14:48:12