我想将五段单独的DNA序列,连成一段完整的DNA序列，可以用overlap-pcr么

mutagenesisbyPCR-drivenoverlapextensioninfo'.2.'][info3]NatureProtocolsinfo'.3.'][info4]2007info'.4.'][info5]NatureProtocols2,924–932(2007)info'.5.'][info6]doi...

PCRvianoveloverlapsequencesinfo'.2.'][info3]InGeneSynthesisMethodsandProtocols,PeccoudJ(ed).Humana:NewYorkinfo'.3.'][info4]2012info'.4.'][info5]97–110info'...

[info1]MatthewD.Nelson,DavidH.A.Fitchinfo'.1.'][info2]OverlapExtensionPCR:AnEfficientMethodforTransgeneConstructioninfo'.2.'][info3]MolecularMethodsfor...

首先引物设计，根据引物设计原则设计了两对引物，我要做overlappcr上臂长度：875bpTC2-S1：5'-ACTCTCGAGATAAGCGCCGTAGGGAAATAC-3'?(Tm=57.8)TC2-S25'-...

目的片段是用overlap方法连接5个片段成功了载体是用反向pcr和酶切线性化的然后用overlap方法构建新载体老是出现图中的条带?试过几次。都是这种情况，条带在点样孔附近。不知道咋办了，求...

载体是用反向pcr扩增的用overlap方法构建新载体老是出现图中的条带试过几次。都是这种情况。不知道咋办了，求各位大神帮帮忙biostar2009飞约疯人院wizardfanyoulinglyw

论文名称：ACombinationStrategyforConstructionof...withOverlapExtensionPCRandOne-StepCloning杂志卷号：JournalofMicrobiologyandBiotechnology,2016;26(12):2184–2191

[info1]GangLiinfo'.1.'][info2]GeneSynthesisMethodBasedonOverlapExtensionPCRandDNAWorksPrograminfo'.2.'][info3]SyntheticBiologyinfo'.3.'][info4]09August2013...

ProlongedOverlapExtension-PCRwithApplicationtothePreparationofLarge-SizeRandomGeneMutagenesisLibraryinEscherichiacoliinfo'.2.'][info3]MethodsMolBiolinfo'...

目标是把重叠PCR获得的一个3kb的回收片段通过TA克隆连接到T载体上。...是overlap回收的PCR片段有问题吗？还是连接时回收片段和载体的比例有问题？求大神指点/(ㄒoㄒ)/~要毕业好着急~！

Fitch,DavidHAinfo'.1.'][info2]OverlapextensionPCR:anefficientmethodfortransgeneconstruction.info'.2.'][info3]Methodsinmolecularbiologyinfo'.3.'][info4]2011...

AssemblyinEscherichiacolibyProlongedOverlapExtensionPCRinfo'.2.'][info3]MethodsinMolecularBiologyinfo'.3.'][info4]2013info'.4.'][info5]1116,183-192info'.5....

mutagenesisbyPCR-drivenoverlapextensioninfo'.2.'][info3]natureprotocolsinfo'.3.'][info4]2007info'.4.'][info5]2,924-932info'.5.'][info7]...

小弟最近在克隆玉米的一个3100大小片段的基因，分为两条1500左右的片段，但是回收两条1500的片段以后，最后一步混合模板，加两端引物的时候，就是P不出3100的目的带，有时候还有一千的杂带...

byPCR-drivenoverlapextensioninfo'.2.'][info3]NatProtoc.2007;2(4):924-32.info'.3.'][info4]NatProtoc.2007;2(4):924-32.info'.4.'][info5]NatProtoc.2007;2(4):924-32....

请问一下大家，Overlap的PCR结果一直没有之前问一些人说Taq酶扩增以后会在后面加PolyA影响overlap效果请问这个影响真的很大吗？大家做overlap都用的什么酶？

DNAAssemblyinEscherichiacolibyProlongedOverlapExtensionPCRinfo'.2.'][info3]DNACloningandAssemblyMethodsinfo'.3.'][info4]2014info'.4.'][info5]Volume1116,...

[info2]Site-SaturationMutagenesisbyOverlapExtensionPCRinfo'.2.'][info3]DirectedEvolutionLibraryCreationMethodsinMolecularBiologyinfo'.3.'][info4]2014info'.4....

mutagenesisusingoverlapextensionPCRinfo'.2.'][info3]InVitroMutagenesisProtocolsinfo'.3.'][info4]1996,info'.4.'][info5]Volume57,pp177-191info'.5.']

[info1]AntonBryksin,IchiroMatsumurainfo'.1.'][info2]OverlapExtensionPCRCloninginfo'.2.'][info3]SyntheticBiology，MethodsinMolecularBiologyinfo'.3.'][info4]09Aug...