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Hydrogen sulfide donor sodium hydrosulfide-induced heat tolerance in tobacco (Nicotiana tabacum L) suspension cultured cells and involvement of Ca2+ and calmodulin

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Hydrogen sulfide donor sodium hydrosulfide-induced heat tolerance in tobacco (Nicotiana tabacum L) suspension cultured cells and involvement of Ca2+ and calmodulin
×÷Õß:Li, ZG (Li, Zhong-Guang)[ 1 ] ; Gong, M (Gong, Ming)[ 1 ] ; Xie, H (Xie, Hong)[ 1 ] ; Yang, L (Yang, Lan)[ 1 ] ; Li, J (Li, Jing)[ 1 ]
PLANT SCIENCE
¾í: 185  Ò³: 185-189
DOI: 10.1016/j.plantsci.2011.10.006
³ö°æÄê: APR 2012
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Hydrogen sulfide (H2S) is considered as a new emerging cell signal in higher plants. Hydrogen sulfide donor, sodium hydrosulfide, pretreatment significantly increased survival percentage of tobacco suspension cultured cells under heat stress and regrowth ability after heat stress, and alleviated decrease in vitality of cells, increase in electrolyte leakage and accumulation of malondialdehyde (MDA). In addition, sodium hydrosulfide-induced heat tolerance was markedly strengthened by application of exogenous Ca2+ and its ionophore A23187, respectively, while this heat tolerance was weakened by addition of Ca2+ chelator ethylene glycol-bis(b-aminoethylether)-N,N,N',N'-tetraacetic acid (EGTA), plasma membrane channel blocker lap, as well as calmodulin (CaM) antagonists chlorpromazine (CPZ) and trifluoperazine (TFP), respectively, but intracellular channel blocker ruthenium red (RR) did not. These results suggested that sodium hydrosulfide pretreatment could improve heat tolerance in tobacco suspension cultured cells and the acquisition of this heat tolerance requires the entry of extracellular Ca2+ into cells across the plasma membrane and the mediation of intracellular CaM. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
¹Ø¼ü´Ê
×÷Õ߹ؼü´Ê:Calcium; Calmodulin; Heat tolerance; Sodium hydrosulfide; Tobacco suspension cells
KeyWords Plus:WHEAT SEED-GERMINATION; CYTOSOLIC CALCIUM; GUARD-CELL; SEEDLINGS; TOXICITY; STRESS; THERMOTOLERANCE; PLANTS
×÷ÕßÐÅÏ¢
ͨѶ×÷ÕßµØÖ·: Li, ZG (ͨѶ×÷Õß)
ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ        Yunnan Normal Univ, Sch Life Sci, Engn Res Ctr Sustainable Dev & Utilizat Biomass E, Minist Educ,Key Lab Biomass Energy & Environm Bio, Kunming 650092, Yunnan Province, Peoples R China.
µØÖ·:
ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ        [ 1 ] Yunnan Normal Univ, Sch Life Sci, Engn Res Ctr Sustainable Dev & Utilizat Biomass E, Minist Educ,Key Lab Biomass Energy & Environm Bio, Kunming 650092, Yunnan Province, Peoples R China
µç×ÓÓʼþµØÖ·:zhongguang_li@163.com
»ù½ð×ÊÖúÖÂл
»ù½ð×ÊÖú»ú¹¹        ÊÚȨºÅ
Natural Science Fundation of Yunnan Province of China        
2010ZC066
Education Department Foundation of Yunnan Province of China        
09Y0145
²é¿´»ù½ð×ÊÖúÐÅÏ¢   
³ö°æÉÌ
ELSEVIER IRELAND LTD, ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND
Àà±ð / ·ÖÀà
Ñо¿·½Ïò:Biochemistry & Molecular Biology; Plant Sciences
Web of Science Àà±ð:Biochemistry & Molecular Biology; Plant Sciences
ÎÄÏ×ÐÅÏ¢
ÎÄÏ×ÀàÐÍ:Article
ÓïÖÖ:English
Èë²ØºÅ: WOS:000301218300020
PubMed ID: 22325880
ISSN: 0168-9452
ÆÚ¿¯ÐÅÏ¢
¼¨Ð§Ç÷ÊÆ: Essential Science IndicatorsSM
Impact Factor (Ó°ÏìÒò×Ó): Journal Citation Reports®
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