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functional genomics

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2Â¥: Originally posted by ·´Ó¦Á´ at 2016-01-05 21:31:38
You could try this protocol, which is very useful for plant total DNA extraction.

I hope you could improve the CTAB method for Soil microorganism total DNA extraction by drawing lessons from this  ...

Thank you ,i will give it a try
4Â¥2016-01-06 14:50:51
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
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You could try this protocol, which is very useful for plant total DNA extraction.

I hope you could improve the CTAB method for Soil microorganism total DNA extraction by drawing lessons from this protocol.

3% CTAB DNA extracion protocol
(modified from Zeng et al. 2002, ACta Botanica Sinica 44; 694-697)

1. washing out polysaccharides with CTAB-free buffer from homogenate.
2. increasing the percentage of CTAB in extraction medium.
3. using high concentration salts pior to DNA precipitation with isopanol.

ÈçºÎÌá¸ßÊÖÌáÍÁÈÀDNAµÄ´¿¶È
2Â¥2016-01-05 21:31:38
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

xiaoqiao_mao

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ÃÕÎíxiaoxiao: ½ð±Ò+10, ¡ïÓаïÖú 2016-01-06 14:51:48
MP FastDNA for soil ÊÔ¼ÁºÐ£¬ÎÒÃÇʵÑéÊÒÒ»Ö±ÓÃÕâ¸ö£¬Ð§¹ûºÜºÃ£¬Ò²Ìá¹ýʪµØµÄDNA¡£Äã¿ÉÒÔÊÔһϡ£
3Â¥2016-01-06 14:36:27
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ÒýÓûØÌû:
3Â¥: Originally posted by xiaoqiao_mao at 2016-01-06 14:36:27
MP FastDNA for soil ÊÔ¼ÁºÐ£¬ÎÒÃÇʵÑéÊÒÒ»Ö±ÓÃÕâ¸ö£¬Ð§¹ûºÜºÃ£¬Ò²Ìá¹ýʪµØµÄDNA¡£Äã¿ÉÒÔÊÔһϡ£

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5Â¥2016-01-06 14:51:22
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