| 查看: 679 | 回复: 10 | |||
| 当前主题已经存档。 | |||
| 【有奖交流】积极回复本帖子，参与交流，就有机会分得作者 lzxiaozi 的 83 个金币 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
lzxiaozi金虫 (小有名气)
|
[交流]
谁有辣根过氧化物酶测过氧化氢含量的文献，重奖100个金币
|
||
| 谁有辣根过氧化物酶测过氧化氢含量的文献？有其他好的方法另有重奖，绝不吝啬,支持我的帖子也奖励啊 |
回复此楼» 猜你喜欢
材料与化工（0856）304求B区调剂
已经有6人回复
-
348求调剂
已经有5人回复
-
308求调剂
已经有5人回复
-
299求调剂
已经有6人回复
-
0856，材料与化工321分求调剂
已经有3人回复
-
311求调剂
已经有7人回复
-
085600，材料与化工321分，求调剂
已经有8人回复
-
一志愿华东理工大学081700，初试分数271
已经有7人回复
-
085601 材料工程 313分 求调剂
已经有5人回复
-
22408 359分调剂
已经有3人回复
★ ★ ★ ★
lzxiaozi(金币+2,VIP+0):谢谢
lzxiaozi(金币+2,VIP+0):谢谢
lzxiaozi(金币+2,VIP+0):谢谢
lzxiaozi(金币+2,VIP+0):谢谢
8楼2008-09-27 23:32:46
xxh8372
荣誉版主 (文坛精英)
小木虫微笑研究僧
- 应助: 0 (幼儿园)
- 贵宾: 3.011
- 金币: 30387.2
- 散金: 371
- 红花: 27
- 沙发: 73
- 帖子: 34233
- 在线: 974.7小时
- 虫号: 248039
- 注册: 2006-05-03
- 专业: 生物化学
- 管辖: 休闲灌水
2楼2008-09-27 23:11:29
xxh8372
荣誉版主 (文坛精英)
小木虫微笑研究僧
- 应助: 0 (幼儿园)
- 贵宾: 3.011
- 金币: 30387.2
- 散金: 371
- 红花: 27
- 沙发: 73
- 帖子: 34233
- 在线: 974.7小时
- 虫号: 248039
- 注册: 2006-05-03
- 专业: 生物化学
- 管辖: 休闲灌水
|
Rhodazine D O2/H2O2 assay. O2 and H2O2 concentrations were determined by the Rhodazine D assay (RDA) (CHEMetrics, Calverton, Virginia, United States). All Quick-Seal centrifuge tubes containing cells were centrifuged at 4 8C (2,000 3 g, 10 min) after irradiation. Supernatants were tested by the RDA. The RDA is suitable for measuring 0.1–1.0 mg/l O2, and 1–10 3 105 M H2O2. Mn(III,IV) are oxidants that will also cause a positive RDA test result. However, Mn(III,IV) dioxides are insoluble in water (circa-neutral pH) and readily removed from suspension by centrifugation (2,0003g, 5 min) or standing. RDA results indicating 1 mg/l or more O2 or 103105 M or greater H2O2 were also tested by the Indigo Carmine assay (CHEMetrics), which yields a blue color suitable for measuring higher concentrations of O2 and H2O2. Once a test solution had filled an assay vial, the open end was sealed anaerobically with vacuum grease, and the vial was stored in the dark. |
3楼2008-09-27 23:17:04
4楼2008-09-27 23:22:38
