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xxh8372
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2楼2008-09-27 23:11:29
xxh8372
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Rhodazine D O2/H2O2 assay. O2 and H2O2 concentrations were determined by the Rhodazine D assay (RDA) (CHEMetrics, Calverton, Virginia, United States). All Quick-Seal centrifuge tubes containing cells were centrifuged at 4 8C (2,000 3 g, 10 min) after irradiation. Supernatants were tested by the RDA. The RDA is suitable for measuring 0.1–1.0 mg/l O2, and 1–10 3 105 M H2O2. Mn(III,IV) are oxidants that will also cause a positive RDA test result. However, Mn(III,IV) dioxides are insoluble in water (circa-neutral pH) and readily removed from suspension by centrifugation (2,0003g, 5 min) or standing. RDA results indicating 1 mg/l or more O2 or 103105 M or greater H2O2 were also tested by the Indigo Carmine assay (CHEMetrics), which yields a blue color suitable for measuring higher concentrations of O2 and H2O2. Once a test solution had filled an assay vial, the open end was sealed anaerobically with vacuum grease, and the vial was stored in the dark. |
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