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Unlike most transcriptional regulators, E. coli DksA (DksAEc) does not interact with promoter DNA. Its promoter specificity derives from the fact that different promoters are rate limited at different steps in the kinetic mechanism. DksA binds to RNA polymerase (RNAP) in all promoter complexes tested to date and alters the rates of a specific step(s) in the pathway to open complex formation, but it affects transcriptional output only from promoters rate limited at that step(s)¡£ ÓÐÄÄλ´óÏÀÃ÷°×Õâ¶Î»°µÄÒâ˼£¬Çó½âÊÍ£¬·Ç³£¸Ðл£¡ |
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