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[求助]
帮我看看这两句话的翻译有没有问题
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细胞加入Trizol后吹打，室温静置5Min，直接提取RNA或放入-80℃冰箱冻存。待Trizol融化后，加入200ul氯仿/ml Trizol，颠倒混匀（30s），室温放置3Min。 Add Trizol to cells and homogenize the mixture, stand for 5 minutes for extracting RNA at room temperature or store RNA in -80 degrees Celsius refrigerator.Once Tizol melted, add 200ul chloroform/ml Trizol and homogenize(30s),stand for 3 minutes at room temperature,centrifuge at 12,000g for 15 min. |
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OK Add Trizol to cells and lysate the cells by mixing the mixture thoroughly; stand the mixture for 5 minutes and then isolate RNA immediately at room temperature or store the lysates at -80C until further analysis. For stored samples, thaw the lysates completely and add 200 ul of chloroform/ml Trizol. Mix the mixture completely by inversion repeatedly for 30s and stand for 3 minutes at room temperature, followed by centrifugation at 12,000 x g for 15 min. |
8楼2015-12-06 11:45:29
2楼2015-12-05 22:51:45
3楼2015-12-06 09:46:18
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这不是抽提RNA吗，你中文叙述的也太复杂了。英文部分用词不准确：例如混匀和homogenize不是一回事。另外，你的英文写的和你所读过的英文文献应该是有很大出入的。我给你总结一下吧。把你自己写的忘了： Trizol was added to the cells, cell lysates were mixed, stood for 5 min at room temperature and used for RNA extraction immediately or stored at -80C. In the latter case, cell lysates were thawed completely and 200 ul of chloroform/ml Trizol were added. The mixture was mixed thoroughly for 30 s, stood for 3 min at room temperature, and centrifuged for 15 min at 12,000 x g. |
4楼2015-12-06 10:10:40
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