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2Â¥2015-12-05 22:51:45
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2Â¥: Originally posted by ÀîÍØ°î at 2015-12-05 22:51:45
ϸ°ûÖмÓÈëTrizol£¬µ÷ÔÈ»ìºÏÎÔÚÊÒξ²ÖÃ5MinÖ±½ÓÌáÈ¡RNA»ò·ÅÈë-80¡æ±ùÏä¶³´æ¡£´ýTrizolÈÚ»¯ºó£¬¼ÓÈë200ulÂÈ·Â/ml Trizol£¬»ìÔÈ£¨30s£©£¬ÊÒηÅÖÃ3Min£¬·ÅÈëÀëÐÄ·ÖÀë»úÔÚ12,000gÀëÐÄÁ¦Ï¹¤×÷15min.

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Trizol was added to the cells, cell lysates were mixed, stood for 5 min at room temperature and used for RNA extraction immediately or stored at -80C. In the latter case, cell lysates were thawed completely and 200 ul of chloroform/ml Trizol were added. The mixture was mixed thoroughly for 30 s, stood for 3 min at room temperature, and centrifuged for 15 min at 12,000 x g.
4Â¥2015-12-06 10:10:40
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