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PEEK_polymer

新虫 (初入文坛)

[求助] Image J 统计孔尺寸 已有2人参与

请问大神们,Image J 软件怎么计算图片中的泡孔数目和尺寸????万分感谢!!

Image J  统计孔尺寸
6-2.jpg
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dota

木虫 (著名写手)

【答案】应助回帖

引用回帖:
2楼: Originally posted by dota at 2015-11-24 09:36:04
1) Set scale. Enlarge your image, draw a line of the exactly same length to the scale bar. For you image, 81.5 pixel = 20 um)

2) Adjust threshold, which showed reasonable segmentations.

3) Anal ...

several screenshots...
Image J  统计孔尺寸-1
1) set scale.png


Image J  统计孔尺寸-2
2) adjust threshold.png


Image J  统计孔尺寸-3
3) analysis particles.png


Image J  统计孔尺寸-4
4) result.png

3楼2015-11-24 09:38:56
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dota

木虫 (著名写手)

【答案】应助回帖

感谢参与,应助指数 +1
1) Set scale. Enlarge your image, draw a line of the exactly same length to the scale bar. For you image, 81.5 pixel = 20 um)

2) Adjust threshold, which showed reasonable segmentations.

3) Analysis Particles. Based on the visional pore sizes, I would say areas between 100 um^2 and 600 um^2 should be the correct pores.

4) Copy the results into the Excel to calculate the average and the stDev.

The 45 pores were detected out. The pore perimeter is 25.6 +/- 9.8 um. Some pore might be missed due to either the irregular shapes or the low image contrasts (not good segmentations)

Cheers!
2楼2015-11-24 09:36:04
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PEEK_polymer

新虫 (初入文坛)

引用回帖:
3楼: Originally posted by dota at 2015-11-24 09:38:56
several screenshots...

1) set scale.png

2) adjust threshold.png

3) analysis particles.png

4) result.png
...

太感谢了!!还有一个问题,就是阈值要怎么调才算合适?Auto 好像不太对
4楼2015-11-24 10:12:29
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dota

木虫 (著名写手)

引用回帖:
4楼: Originally posted by PEEK_polymer at 2015-11-24 10:12:29
太感谢了!!还有一个问题,就是阈值要怎么调才算合适?Auto 好像不太对...

Slightly adjust, analysis, and check up the result. If not so happy, just re-do. It is based on your personal experience. So, there is bias.
5楼2015-11-24 11:04:46
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