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【答案】应助回帖
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icebergwu: 金币+30, 翻译EPI+1, ★★★★★最佳答案 2015-11-20 11:08:24
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Using ultra-high performance liquid chromatography (UPLC)-high resolution quadrupole time-of flight MS, the discriminant analysis of 5 components of the feed was conducted. Based on C18 chromatogram column separation, the acetonitrile - 0.1% formic acid aqueous solution (including 2mmol•L-1 ammonium acetate) was employed as the mobile phase at flow rate of 0.4mL•min-1 for gradient elution at column temperature of 40℃; Under the mass spectrometer condition of electrospray ionization, and adopting anion full scan mode as detection mode, the identification of 5 components of the feed were implemented based on retention time, exact molecular weight, and secondary mass spectral characteristic fragments. Compared with HPLC, this method is of better application value for its advantages of accurate judgement and rapid detection. |
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