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| Overall strain variability for growth on selective media was lower after 48 h than after 24 h; for each selective media, no significant differences in the detected count was found in any of the 12 isolates tested (ANOVA; all P-values >0.05), although some isolates showed variability among biological replicates. For example, after 48 h, FSL R5-555 and W6-524 each showed no growth for at least one biological replicate on MAC, CPETRI, VRBA, and VRBAO, although for each of these isolates, all 3 biological replicates produced growth on SPCP. This variability among replicates contributed to the low SPCP predictability for these selective media. These findings indicate that each of these isolates may require a longer incubation time than 48 h to reproducibly form colonies on MAC, CPETRI, VRBA, and VRBAO. In contrast, we observed less within-strain variability on CVTAP and CVTAS, as all isolates showed colony formation for all 3 replicates on both media. |
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Overall strain variability for growth on selective media was lower after 48 h than after 24 h; ÓëÅàÑø24СʱºóÏà±È£¬48СʱºóµÄ£¬Ñ¡ÔñÐÔÅàÑø»ùÄÚµÄ×ܾúÖê±ä»¯¸üС¡£ for each selective media, no significant differences in the detected count was found in any of the 12 isolates tested (ANOVA; all P-values >0.05), although some isolates showed variability among biological replicates. ¶ÔÓÚÿ¸öÑ¡ÔñÐÔÅàÑø»ù£¬ ÔÚÕâ12¸ö¶ÀÁ¢ÅàÑøµÄ±»²â¾úÖêÖУ¬²¢Ã»Óз¢ÏÖÃ÷ÏÔµÄ̽²â¼ÆÊýµÄ²»Í¬ (·½²î·ÖÎö; all P-values >0.05)£¬¾¡¹ÜÓÐЩ¶ÀÁ¢ÅàÑø¾úÖêÕ¹ÏÖ³ýÁËÉúÎï¸´ÖÆÉϵıäÒìÐÔ¡£ For example, after 48 h, FSL R5-555 and W6-524 each showed no growth for at least one biological replicate on MAC, CPETRI, VRBA, and VRBAO, although for each of these isolates, all 3 biological replicates produced growth on SPCP. ÀýÈ磬ÅàÑø48Сʱºó£¬ FSL R5-555ºÍ W6-524 ºÅ¾úÖ궼ûÓÐÕ¹ÏÖ³öÄÄÅÂÊÇÒ»ÏîÔÚÅàÑø»ùMAC, CPETRI, VRBA, ºÍVRBAOÄÚµÄÉúÎï¸´ÖÆÐÔ£¬¾¡¹ÜÔÚÕâЩ¶ÀÁ¢ÅàÑø¾úÖêÖУ¬Ò»¹²ÓÐ3¸öÉúÎï¸´ÖÆ²úÉúÁËSPCPµÄÔö³¤¡£ This variability among replicates contributed to the low SPCP predictability for these selective media. These findings indicate that each of these isolates may require a longer incubation time than 48 h to reproducibly form colonies on MAC, CPETRI, VRBA, and VRBAO. ÉúÎï¸´ÖÆµÄ±äÒìÐÔÓÐÖúÓÚ¶ÔÕâЩѡÔñÐÔÅàÑø»ù½øÐеÍSPCPµÄ¿ÉÔ¤²âÐÔ¡£ÕâЩ·¢ÏÖ±íÃ÷ÿ¸ö¶ÀÁ¢ÅàÑøµÄ¾úÖê¶¼¿ÉÄÜÐèÒª¸ü³¤µÄÅàÑøÊ±¼ä£¨²»Ö¹48Сʱ£©£¬´Ó¶øÔÚMAC, CPETRI, VRBA, and VRBAOÅàÑø»ùÖУ¬¿ÉÔÙÉú²úµØÐγɾúÂä¡£ In contrast, we observed less within-strain variability on CVTAP and CVTAS, as all isolates showed colony formation for all 3 replicates on both media. Ïà·´£¬ÎÒÃÇÔÚCVTAPºÍCVTASÅàÑø»ùÄÚ£¬¹Û²ìµ½¸üÉٵľúÖêÄڵıäÒìÐÔ£¬ÒòΪËùÓеĶÀÁ¢ÅàÑø¾úÖ꣬¶¼Õ¹ÏÖ³öÁ˾úÂäµÄÐγɣ¬ÒÔÌåÏÖÔÚ2ÖÐÅàÑø»ùÖеÄËùÓÐ3ÏîÉúÎï¸´ÖÆÐÔ |
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