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中国的_BOY

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After a 48-h incubation, improved detection efficiencies for certain selective media, particularly CVTA, were observed. Therefore, a 48-h incubation time resulted in an overall improvement in detection of Pseudomonas spp. in milk, which is consistent with the 48-h incubation times recommended for CPETRI and CVTA (Frank and Yousef, 2004) but not consistent with the recommended incubation time for MAC, which is 18 to 24 h (Difco, 1998). Our results suggest that plating milk samples on CVTA and incubating plates for 48 h is the best method for detecting Pseudomonas contamination in pasteurized milk. These findings support the method for the enumeration of gram-negative organisms in milk presented in Standard Methods for the Examination of Dairy Products(Frank and Yousef, 2004). However, whereas Standard Methodsrecommends incubating plates at 21°C, our findings suggest that incubating plates at 32°C can accurately detect a diversity of Pseudomonas isolates in milk.

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baoshanqiu

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中国的_BOY: 金币+37, 翻译EPI+1, ★★★★★最佳答案, 谢谢了。 2015-10-30 14:05:47
After a 48-h incubation, improved detection efficiencies for certain selective media, particularly CVTA, were observed. Therefore, a 48-h incubation time resulted in an overall improvement in detection of Pseudomonas spp. in milk, which is consistent with the 48-h incubation times recommended for CPETRI and CVTA (Frank and Yousef, 2004) but not consistent with the recommended incubation time for MAC, which is 18 to 24 h (Difco, 1998).
48-h培养后,一些选择性培养基,特别是CVTA的检测效率有所提高。因此48-h培养整体改善了牛奶中假单胞菌属的检测,这与推荐的CPETRI 和 CVTA (Frank 和 Yousef, 2004)培养48-h一致,但与推荐的MAC培养时间不一致,后者为 18 到 24 h (Difco, 1998)。
3楼2015-10-30 00:49:49
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中国的_BOY: 金币+30, ★★★★★最佳答案, 辛苦了 2015-10-30 14:06:03
After a 48-h incubation, improved detection efficiencies for certain selective media, particularly CVTA, were observed. Therefore, a 48-h incubation time resulted in an overall improvement in detection of Pseudomonas spp.
经过48小时的潜伏期,可以观察到对于某些选择性培养基中,尤其是CVTA培养基中,探测效率有所提高。 因此48小时的潜伏期导致了,在探测假单胞菌时,总体效率的提升

in milk, which is consistent with the 48-h incubation times recommended for CPETRI and CVTA (Frank and Yousef, 2004) but not consistent with the recommended incubation time for MAC, which is 18 to 24 h (Difco, 1998).
对于牛奶培养基, 它与CPETRI 和CVTA培养基一样,推荐进行48小时的潜伏期 (Frank 和 Yousef2004的研究成果),而与MAC培养基不一样,后者是推荐进行18-24小时的潜伏期(Difco 1998年的研究成果)。

Our results suggest that plating milk samples on CVTA and incubating plates for 48 h is the best method for detecting Pseudomonas contamination in pasteurized milk.
我们的研究结果标明,在CVTA培养基中板式乳样和进行板式培养48小时,是探测巴氏杀菌奶中假单胞菌污染的最佳方法。


These findings support the method for the enumeration of gram-negative organisms in milk presented in Standard Methods for the Examination of Dairy Products(Frank and Yousef, 2004).
这些研究结果,支持运用标准方法检测乳制品中,列举牛奶培养基中革兰氏阴性的有机体的方法。

However, whereas Standard Methods recommends incubating plates at 21°C, our findings suggest that incubating plates at 32°C can accurately detect a diversity of Pseudomonas isolates in milk.。
然而,标准方法建议在21摄氏度下进行板式培养,而我们的研究结果建议在32摄氏度下进行板式培养,后者能准确探测出牛奶培养基中各种假单胞菌独立菌落。
2楼2015-10-29 21:52:14
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baoshanqiu

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Our results suggest that plating milk samples on CVTA and incubating plates for 48 h is the best method for detecting Pseudomonas contamination in pasteurized milk. These findings support the method for the enumeration of gram-negative organisms in milk presented in Standard Methods for the Examination of Dairy Products(Frank and Yousef, 2004). However, whereas Standard Methodsrecommends incubating plates at 21°C, our findings suggest that incubating plates at 32°C can accurately detect a diversity of Pseudomonas isolates in milk.
我们的结果表明在CVTA上接种牛奶样品并将平皿培养 48 h是检测巴氏消毒牛奶中假单胞菌污染的最佳方法。这些发现与乳制品检查的标准方法(Frank 和 Yousef, 2004)中出现的牛奶中革兰氏阴性菌计数法相一致。然而,标准法推荐在21°C培养平皿,我们发现在32°C培养平皿能准确检出牛奶中各种假单胞菌分离物。
4楼2015-10-30 01:05:31
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