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After a 48-h incubation, improved detection efficiencies for certain selective media, particularly CVTA, were observed. Therefore, a 48-h incubation time resulted in an overall improvement in detection of Pseudomonas spp. in milk, which is consistent with the 48-h incubation times recommended for CPETRI and CVTA (Frank and Yousef, 2004) but not consistent with the recommended incubation time for MAC, which is 18 to 24 h (Difco, 1998). Our results suggest that plating milk samples on CVTA and incubating plates for 48 h is the best method for detecting Pseudomonas contamination in pasteurized milk. These findings support the method for the enumeration of gram-negative organisms in milk presented in Standard Methods for the Examination of Dairy Products(Frank and Yousef, 2004). However, whereas Standard Methodsrecommends incubating plates at 21¡ãC, our findings suggest that incubating plates at 32¡ãC can accurately detect a diversity of Pseudomonas isolates in milk.
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After a 48-h incubation, improved detection efficiencies for certain selective media, particularly CVTA, were observed. Therefore, a 48-h incubation time resulted in an overall improvement in detection of Pseudomonas spp.
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in milk, which is consistent with the 48-h incubation times recommended for CPETRI and CVTA (Frank and Yousef, 2004) but not consistent with the recommended incubation time for MAC, which is 18 to 24 h (Difco, 1998).
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Our results suggest that plating milk samples on CVTA and incubating plates for 48 h is the best method for detecting Pseudomonas contamination in pasteurized milk.
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These findings support the method for the enumeration of gram-negative organisms in milk presented in Standard Methods for the Examination of Dairy Products(Frank and Yousef, 2004).
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However, whereas Standard Methods recommends incubating plates at 21¡ãC, our findings suggest that incubating plates at 32¡ãC can accurately detect a diversity of Pseudomonas isolates in milk.¡£
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ÖйúµÄ_BOY: ½ð±Ò+37, ·­ÒëEPI+1, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸, ллÁË¡£ 2015-10-30 14:05:47
After a 48-h incubation, improved detection efficiencies for certain selective media, particularly CVTA, were observed. Therefore, a 48-h incubation time resulted in an overall improvement in detection of Pseudomonas spp. in milk, which is consistent with the 48-h incubation times recommended for CPETRI and CVTA (Frank and Yousef, 2004) but not consistent with the recommended incubation time for MAC, which is 18 to 24 h (Difco, 1998).
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Our results suggest that plating milk samples on CVTA and incubating plates for 48 h is the best method for detecting Pseudomonas contamination in pasteurized milk. These findings support the method for the enumeration of gram-negative organisms in milk presented in Standard Methods for the Examination of Dairy Products(Frank and Yousef, 2004). However, whereas Standard Methodsrecommends incubating plates at 21¡ãC, our findings suggest that incubating plates at 32¡ãC can accurately detect a diversity of Pseudomonas isolates in milk.
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