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| After a 48-h incubation, improved detection efficiencies for certain selective media, particularly CVTA, were observed. Therefore, a 48-h incubation time resulted in an overall improvement in detection of Pseudomonas spp. in milk, which is consistent with the 48-h incubation times recommended for CPETRI and CVTA (Frank and Yousef, 2004) but not consistent with the recommended incubation time for MAC, which is 18 to 24 h (Difco, 1998). Our results suggest that plating milk samples on CVTA and incubating plates for 48 h is the best method for detecting Pseudomonas contamination in pasteurized milk. These findings support the method for the enumeration of gram-negative organisms in milk presented in Standard Methods for the Examination of Dairy Products(Frank and Yousef, 2004). However, whereas Standard Methodsrecommends incubating plates at 21¡ãC, our findings suggest that incubating plates at 32¡ãC can accurately detect a diversity of Pseudomonas isolates in milk. |
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After a 48-h incubation, improved detection efficiencies for certain selective media, particularly CVTA, were observed. Therefore, a 48-h incubation time resulted in an overall improvement in detection of Pseudomonas spp. in milk, which is consistent with the 48-h incubation times recommended for CPETRI and CVTA (Frank and Yousef, 2004) but not consistent with the recommended incubation time for MAC, which is 18 to 24 h (Difco, 1998). 48-hÅàÑøºó£¬Ò»Ð©Ñ¡ÔñÐÔÅàÑø»ù£¬ÌرðÊÇCVTAµÄ¼ì²âЧÂÊÓÐËùÌá¸ß¡£Òò´Ë48-hÅàÑøÕûÌå¸ÄÉÆÁËÅ£ÄÌÖмٵ¥°û¾úÊôµÄ¼ì²â£¬ÕâÓëÍÆ¼öµÄCPETRI ºÍ CVTA (Frank ºÍ Yousef, 2004)ÅàÑø48-hÒ»Ö£¬µ«ÓëÍÆ¼öµÄMACÅàÑøÊ±¼ä²»Ò»Ö£¬ºóÕßΪ 18 µ½ 24 h (Difco, 1998)¡£ |
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Our results suggest that plating milk samples on CVTA and incubating plates for 48 h is the best method for detecting Pseudomonas contamination in pasteurized milk. These findings support the method for the enumeration of gram-negative organisms in milk presented in Standard Methods for the Examination of Dairy Products(Frank and Yousef, 2004). However, whereas Standard Methodsrecommends incubating plates at 21¡ãC, our findings suggest that incubating plates at 32¡ãC can accurately detect a diversity of Pseudomonas isolates in milk. ÎÒÃǵĽá¹û±íÃ÷ÔÚCVTAÉϽÓÖÖÅ£ÄÌÑùÆ·²¢½«Æ½ÃóÅàÑø 48 hÊǼì²â°ÍÊÏÏû¶¾Å£ÄÌÖмٵ¥°û¾úÎÛȾµÄ×î¼Ñ·½·¨¡£ÕâЩ·¢ÏÖÓëÈéÖÆÆ·¼ì²éµÄ±ê×¼·½·¨(Frank ºÍ Yousef, 2004)ÖгöÏÖµÄÅ£ÄÌÖиïÀ¼ÊÏÒõÐÔ¾ú¼ÆÊý·¨ÏàÒ»Ö¡£È»¶ø£¬±ê×¼·¨ÍƼöÔÚ21¡ãCÅàÑøÆ½Ãó£¬ÎÒÃÇ·¢ÏÖÔÚ32¡ãCÅàÑøÆ½ÃóÄÜ׼ȷ¼ì³öÅ£ÄÌÖи÷ÖÖ¼Ùµ¥°û¾ú·ÖÀëÎï¡£ |
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