| 查看: 662 | 回复: 1 | |||
| 【悬赏金币】回答本帖问题，作者杀神一刀斩10将赠送您 6 个金币 | |||
[求助]
求翻译 实验 已有1人参与
|
|||
|
oreYouBegin PrecautionsNLysis/BindingBufferandWashBufferIcontainguanidinehydrochloride whichisanirritant.Alwayswearglovesandfollowstandardsafetypre- cautionstominimizecontactwhenhandling. ?Donotletthesebufferstouchyourskin,eyes,ormucousmembranes.If contactdoesoccur,washtheaffectedareaimmediatelywithlargeamounts ofwater;otherwise,thereagentmaycauseburns.Ifyouspillthereagent, dilutethespillwithwaterbeforewipingitup. ?NeverstoreorusetheBindingBuffernearhumanoranimalfood. ?Alwayswearglovesandfollowstandardsafetyprecautionswhenhandling thesebuffers. SampleMaterialSolidtissue(e.g.,mouseliver,spleen,lung,heart),1–10mg(formortar/pestle disruption)or1–25mg(forrotor-statorhomogenization). Handlingand Storageof StartingMaterial ToavoidanydegradationofRNAinsamplematerialbyintracellularRNases followtheseguidelines: ?Processthesamplematerialassoonascollected ?Ifstorageofsamplematerialisrequired,flashfreezeinliquidnitrogenand storeat-60°C NDonotallowfrozentissuematerialtothawduringhandling(e.g., weighing).Therelevantproceduresshouldbecarriedoutasquicklyas possible. ?Samplescanalsobestoredat-60°CorbelowinLysis/BindingBufferafter disruptionandhomogenization. LYieldsmayvarydependingonstoragetime. NUsesteriledisposablepolypropylenetubesandtipsinordertoavoid RNasecontamination.Wearglovesduringtheassay. Disruptionand Homogenization ?Efficientdisruptionandhomogenizationofthestartingmaterialisessential forintra-cellularRNAisolationproceduresfromtissues. ?Thecompletedisruptionofcellwallsandplasmamembranesofcellsand organellesisabsolutelyrequiredtoreleasealltheRNAcontainedinthesam- ple.Incompletedisruptionresultsinsignificantlyreducedyields. ?Homogenizationisnecessarytoreducetheviscosityofthecelllysatespro- ducedbydisruption.Homogenizationshearsthehighmolecularweight genomicDNAandotherhighmolecularweightcellularcomponentstocre- ateahomogeneouslysate. ?Incompletehomogenizationresultsinsignificantlyreducedyields.Somedis- ruptionmethodssimultaneouslyhomogenizethesample,whileothers requireanadditionalhomogenizationst 发自小木虫Android客户端 |
回复此楼» 猜你喜欢
有多少人是今天查系统知道结果的？
已经有19人回复
-
国社科又开始会评了，不知道这次命运如何
已经有9人回复
-
能否申诉？
已经有5人回复
-
为什么到现在没收到通知？
已经有5人回复
-
有没有仍没收到信息的
已经有8人回复
-
售SCI一区文章，我:8O5.5.1.O5.4,科目全,可伽急
已经有5人回复
-
售SCI一区文章，我:8O5.5.1.O5.4,科目全,可伽急
已经有4人回复
-
售SCI一区T0P文章，我:8.O.55.1.O54,科目全,可伽急
已经有4人回复
-
售SCI文章，我:8O5.5.1.O.54,科目齐全,可+急
已经有5人回复
-
售SCI一区文章，我:8.O.55.1.O.54,科目齐全,可伽急
已经有4人回复
sqlg888
铁虫 (正式写手)
- SFL-EPI: 1
- 应助: 163 (高中生)
- 金币: 4544.2
- 红花: 27
- 帖子: 995
- 在线: 191.8小时
- 虫号: 4176864
- 注册: 2015-10-27
- 专业: 民族药学
2楼2015-11-14 16:44:25
