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中国的_BOY

木虫 (著名写手)

我是瞎说的

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[求助] 求翻译这段话

Frozen stocks of the bacterial isolates were obtained from the Cornell University Food Safety Laboratory (CU-FSL) isolate collection. Cultures were streaked from −80°C frozen stock onto brain heart infusion (Difco, Sparks, MD) agar and incubated for 18 h at 32°C. A colony from each strain was used to inoculate 5 mL of sterile brain heart infusion broth in 16-mm glass test tubes, which were then incubated for 48 h with aeration (shaking at 220 rpm) at 32°C. A 1-mL aliquot of the culture was then centrifuged at 14,000 × gand the supernatant was removed. The resulting cell pellet was resuspended in 1 mL of phosphate dilution buffer (Weber Scientific, Trenton, NJ), diluted to 1,000 to 10,000 cfu/mL, and inoculated into commercially purchased UHT pasteurized milk (1% milk; Organic Valley, La Farge, WI) for an approximate inoculum of 2 to 3 log cfu/mL. Inocula were enumerated by spiral plating the appropriate dilution on SPC agar (EMD, Gibbstown, NJ), followed by incubation at 32°C for 24 to 48 h. Inoculated UHT milk was incubated at 6°C for 48 h to simulate refrigerated storage conditions. After a 48-h incubation at 6°C, appropriate dilutions of inoculated UHT samples prepared in phosphate dilution buffer were plated in duplicate on (1) SPC pour plates (SPCP); (2) SPC plates inoculated by spiral plating (SPCS); (3) CVTA [plate count agar (EMD, Gibbstown, NJ) with 2,3,5-triphenyltetrazolium chloride (Sigma-Aldrich, St. Louis, MO)] pour plates (CVTAP); (4) CVTA plates inoculated by spiral plating (CVTAS); (5) VRBA plates inoculated by pour plating (Difco, Sparks, MD); (6) VRBA with an overlay of approximately 5 mL of VRBA agar (VRBAO); (7) CPETRI (3M); or (8) MAC (Difco) inoculated by spiral plating. All media were incubated at 32°C for 48 h, with enumeration after 24 and 48 h. Three biological replications of the experiment were completed.

题目：评估一下可用于检测巴氏杀菌乳中假单胞菌属种类的各种选择性培养基

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也不是不快乐。只是容易意识到痛苦也不远了。

1楼 2015-10-21 22:21:09

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

baoshanqiu

至尊木虫 (著名写手)

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专业: 胃肠道免疫相关疾病

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中国的_BOY: 金币+100, 翻译EPI+1, ★★★★★最佳答案, 谢谢啊，翻译的不错 2015-10-23 09:01:57

细菌分离物的冻存原种来自于康奈尔大学食品安全实验室(CU-FSL) 菌种保存。将培养物自−80°C冻存原种划线接种于脑心浸液(Difco, Sparks, MD)琼脂上32°C培养18 h。每一株选一个菌落接种于无菌16-mm玻璃试管内的5ml脑心浸液肉汤，置 32°C通气（220 rpm振摇）培养48h。取每份1ml培养物于14,000 × g离心、去上清。所得细胞沉淀物用1ml磷酸盐稀释缓冲液(Weber Scientific, Trenton, NJ)再悬浮，稀释的 1,000 至10,000 cfu/mL,以大约2 to 3 log cfu/mL量接种于市售超高温巴氏消毒的牛奶(1% 牛奶; Organic Valley, La Farge, WI)。培养菌液计数以适当稀释度于SPC（标准平板计数）琼脂 (EMD, Gibbstown, NJ)上做旋转平板涂布，32°C 培养 24至 48 h 。接种了细菌的超高温消毒牛奶置6°C 孵育48 h模拟冰箱贮存条件。接种了细菌的超高温消毒牛奶样品经6°C 孵育48 h后，用磷酸盐稀释缓冲液适当稀释，双份接种于（1） SPC倾注平板 (SPCP); （2）旋转平板涂布接种SPC (SPCS); (3)CVTA（结晶紫四氮唑） [平板计数琼脂(EMD, Gibbstown, NJ) 含 2,3,5-氯化三苯四氮唑 (Sigma-Aldrich, St. Louis, MO)] 倾注平板(CVTAP); (4) 旋转平板涂布接种CVTA(CVTAS); (5)VRBA（紫红胆汁琼脂）倾注接种平板 (Difco, Sparks, MD); (6) 以大约5mlVRBA覆盖VRBA琼脂 (VRBAO); (7)CPETRI (大肠杆菌皿膜） (3M);或(8) 旋转平板涂布接种MAC（麦康凯）(Difco)。所有培养基置32°C 培养48 h，于24和48h后计数。实验进行了3次生物学重复。