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1¡¢In the gut, we observed significant reduction of Th17 cells in lamina propria lymphocytes (LPL) of small intestine in adultATB mice compared with controls , consistent with the profile of IL-17 in the skin .
2¡¢Furthermore, the frequencies of IL-22t T cells and IL-22tab T cells were both significantly decreased in adult ATB, compared with control mice without antibiotic treatment .
3¡¢Decrease of Th17 cells corresponded to the decrease in the expression of CCR6 in TCRbtCD4t T cells in the gut; a chemokine receptor required for migration of gut Th17 cells to the skin inflammatory tissues21 .
4¡¢Thus, we have demonstrated that the decrease in IL-22t and IL-17t T cells in the skin is likely a primary factor that accounts for the amelioration of the skin disease in mice depleted of gut bacteria.
5.At the phylum level, we have observed decreased abundance of Bacteroidetes (Kruskal¨CWallis test, P=0.04953), Actinobacteria (Kruskal¨C Wallis test, P=0.0463) and Cyanobacteria (Kruskal¨CWallis test, P=0.0369) in adultATB mice .
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1¡¢In the gut, we observed significant reduction of Th17 cells in lamina propria lymphocytes (LPL) of small intestine in adult ATB mice compared with controls , consistent with the profile of IL-17 in the skin.
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2¡¢Furthermore, the frequencies of IL-22t T cells and IL-22tab T cells were both significantly decreased in adult ATB, compared with control mice without antibiotic treatment.
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3¡¢Decrease of Th17 cells corresponded to the decrease in the expression of CCR6 in TCRbtCD4t T cells in the gut; a chemokine receptor required for migration of gut Th17 cells to the skin inflammatory tissues.
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4¡¢Thus, we have demonstrated that the decrease in IL-22t and IL-17t T cells in the skin is likely a primary factor that accounts for the amelioration of the skin disease in mice depleted of gut bacteria.
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5¡¢At the phylum level, we have observed decreased abundance of Bacteroidetes (Kruskal¨CWallis test, P=0.04953), Actinobacteria (Kruskal¨C Wallis test, P=0.0463) and Cyanobacteria (Kruskal¨CWallis test, P=0.0369) in adult ATB mice.
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lamina propria lymphocytes (LPL)£º¹ÌÓвãÁܰÍϸ°û£»
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