| 查看: 819 | 回复: 12 | |||
| 当前只显示满足指定条件的回帖,点击这里查看本话题的所有回帖 | |||
[交流]
Nanomaterials in fluorescence-based biosensing文献翻译,欢迎交流
|
|||
|
看到一篇关于荧光材料的好文章,希望自己有毅力翻译出来。有翻译错误的地方欢迎指出。 Anal Bioanal Chem (2009) 394:47–59 DOI 10.1007/s00216-009-2643-x Nanomaterials in fluorescence-based biosensing Wenwan Zhong Wenwan Zhong has been Assistant Professor of Chemistry at the University of California, Riverside, since July 2006. She received the Pilot In- terdisciplinary Research Award from the Institute for Integrative Genome Biology of UC, River-side. Her current research in- terests are: developing novel analytical strategies for utilizing nanomaterials in biosensing; studying nanotoxicity using mi- croscale separation techniques like capillary electrophoresis; and developing field-flow fractionation-based methods for purification and analysis of large protein complexes. |
» 本帖附件资源列表
-
欢迎监督和反馈:小木虫仅提供交流平台,不对该内容负责。
本内容由用户自主发布,如果其内容涉及到知识产权问题,其责任在于用户本人,如对版权有异议,请联系邮箱:xiaomuchong@tal.com - 附件 1 : Fluorescence.pdf
2015-08-16 22:50:46, 564.83 K
» 猜你喜欢
美国南加大(USC)药学院Prof. Yong (Tiger) Zhang课题组诚聘博士后
已经有4人回复
反应产物总提不上量?聊聊无机焦磷酸酶在核酸合成里的实用价值
已经有0人回复
生物力学与组织工程学论文润色/翻译怎么收费?
已经有174人回复
【经验分享】体外转录踩坑无数,挖到一款好用的国产 T7 RNA 聚合酶
已经有0人回复
体外转录 / PCR 总提不上产量?终于找到稳定好用的无机焦磷酸酶
已经有5人回复
[会议预告]2026生物医药产业发展大会
已经有0人回复
【产品测评分享】体外转录产量不理想、dsRNA 副产物高?T7 RNA 聚合酶实测体验
已经有4人回复
【交流】蛋白标签切除踩坑不断?聊聊 TEV 蛋白酶实操体会
已经有0人回复
【交流】SUMO 融合蛋白切标签踩坑?聊聊 SUMO 蛋白酶实操体会
已经有1人回复
» 本主题相关商家推荐: (我也要在这里推广)
» 抢金币啦!回帖就可以得到:
告别流水线爽文!108万字完结仙侠《女娲石之灵石奇缘(仙界篇)》值得细品
+1/9040
散金祈福
+5/4105
散金祈福
+5/1315
散金祈福
+3/837
华西生物制药研究院招聘科研助理
+2/406
Polymers 期刊特刊 组稿
+1/80
焦虑
+1/78
哈尔滨工业大学(校本部)电子与信息工程学院2027级博士招生
+1/66
面上基金祈福
+1/45
瑞典-博后-固态电池-粘结剂-锂离子电池方向
+1/37
流放岭南浮肿了求救
+1/26
pcold 原核表达载体
+1/11
中科院大连化学物理研究所 招聘 催化剂研发方向 科研助理 2名
+1/6
深圳大学特聘教授课题组专任教师及博士后招聘
+1/2
北理工集成电路杰青团队 | 诚招科助理
+1/2
找女友
+1/2
出全新蔡司 Plan?APOCHROMAT 20×/0.8 平场复消色差物镜
+1/2
清华大学环境课题组招聘环境工作专业客座生研究生2-3名(应用导向)
+1/1
Olink高通量蛋白组测序:PEA 技术驱动的微量样本蛋白全景解析
+1/1
【产品测评分享】体外转录产量不理想、dsRNA 副产物高?T7 RNA 聚合酶实测体验
+1/1
8楼2015-08-16 23:47:51
★ ★ ★ ★ ★
红舟流星: 金币+5, 欢迎来生物材料板块积极交流 2015-08-18 09:43:06
红舟流星: 金币+5, 欢迎来生物材料板块积极交流 2015-08-18 09:43:06
|
Abstract Fluorescence-based detection is the most common method utilized in biosensing because of its high sensitivity, simplicity, and diversity. In the era of nanotechnology, nanomaterials are starting to replace traditional organic dyes as detection labels because they offer superior optical properties, such as brighter fluorescence, wider selections of excitation and emission wavelengths, higher photostability, etc. Their size- or shape-controllable optical characteristics also facilitate the selection of diverse probes for higher assay throughput. Furthermore, the nanostructure can provide a solid support for sensing assays with multiple probe molecules attached to each nanostructure, simplifying assay design and increasing the labeling ratio for higher sensitivity. The current review summarizes the applications of nanomaterials—including quantum dots, metal nanoparticles, and silica nanoparticles—in biosensing using detection techniques such as fluorescence, fluorescence resonance energy transfer (FRET), fluorescence lifetime measurement, and multiphoton microscopy. The advantages nanomaterials bring to the field of biosensing are discussed. The review also points out the importance of analytical separations in the preparation of nanomaterials with fine optical and physical properties for biosensing. In conclusion, nanotechnology provides a great opportunity to analytical chemists to develop better sensing strategies, but also relies on modern analytical techniques to pave its way to practical applications. 因其高灵敏度、简单和多样,基于荧光的检测是生物传感中最普遍的方法。在纳米技术时代,纳米材料(nanomaterials)开始代替用于检测标记(detection label)的传统有机染料。纳米材料具有优良的光学性质(optical property),如更明亮的荧光(brighter fluorescence)、更宽的激发和发射波长、更高的光稳定性(photostability)等。纳米材料尺寸和形状可控的光学特征(size- or shape-controlling optical characteristics)方便了用于高通量检测的不同探针的选择(facilitate the selection of diverse probes for higher assay throughput)。此外,纳米结构可以为传感分析(sensing assay)提供固相支持,每个纳米结构上带有多重探针分子,简化了检测的设计,增加了标记效率,从而获得了更高的灵敏度。本综述对生物传感领域应用荧光、荧光共振能量转移(fluorescence resonance energy transfer,FRET)、荧光寿命测量(fluorescence lifetime measurement)、多光子显微镜等检测技术的纳米材料(包括量子点(quantum dots)、金属纳米颗粒(metal nanoparticles)、二氧化硅纳米颗粒)的应用进行了总结。讨论了纳米材料对该领域所带来的优势。本文也指出了制备具有优异光学和物理性能的纳米材料时分析分离(analytical separations)的重要性。综上所述,纳米技术为分析化学家提供了极大的机遇来开发更好的传感方法,但其实际应用也依赖于现代分析技术的推动。 |
2楼2015-08-16 22:55:22
5楼2015-08-16 23:19:28
简单回复
2015-08-16 23:26
回复
snoopyzxx(金币+1): 谢谢参与


2015-08-16 23:55
回复


假大空11楼
2015-08-17 00:00
回复
snoopyzxx(金币+1): 谢谢参与











回复此楼