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首先你应该了解清楚大鼠脑组织中的海马的解剖结构。
而且不同的目的取法是不一样，比如你做神经细胞培养，是去新生或胚胎鼠，比较困难，成年的比较容易。如果是进行放射免疫学分析，可以是用热水煮过的组织，很容易分离，结构也很清楚。请你注意脑组织分离的原则：钝性分离，可以推，可以拉，不可以切。
step 1:This is a dorsal view of a mouse’s skull, in order to access the brain you can
cut along the coronal suture and sagittal suture then pull off both sides of parietal
bone and interparietal bone
step 2: Now the skull is open and you can see the brain clearly, in order to get the
hippocampus you’d better keep the brain in the cranial cavity so the brain will not
move when you continue with the following steps
step 3: In order to expose the hippocampus you need to remove the cerebral cortex
covering it. The first incision is at the end of the hemisphere; the incision should be
about 0.7mm deep for most adult mouse that you might not hurt the hippocampus
while to expose it. The 2nd incision is about 1.5-2mm in front of the first one, this
incision you need cut into the lateral ventricle, both of the incisions go to the ventral
of the brain and meet there. Now this piece of cortex is free, pull it up, you will see
the hippocampus just like in this picture, also you can see the CSF in the opened
ventricle.
step 4: Keep working on the other side of the brain pull up both sides of the cortex
that covering the hippocampus along the ventricle. Now you can see the dorsal part
of the hippocampus. Separate the rest of the hippocampus from the cortex covering
it along the surface of the hippocampus towards the ventral part of the hippocampus.
step 5: Keep working on the other side of the brain pull up both sides of the cortex
that covering the hippocampus along the ventricle. Now you can see the dorsal part
of the hippocampus. Separate the rest of the hippocampus from the cortex covering
it along the surface of the hippocampus towards the ventral part of the hippocampus.
step 6: Now you need to free the hippocampus from the surrounding tissue
step 7: Here is the hippocampus picked out from the brain. |
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