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bio_yifan

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[交流] 2026 applications for research assistant positions

(1)EDUCATION
Sep. 2023 - present            Fujian Agricultural and Forestry University             Biomedical Science  
Core Courses (with scores): Bioinformatics (89), Research Progress in Biotechnology and Pharmaceuticals (92), Comprehensive Experiment of Molecular Biology (91), Molecular Biology and Genetic Engineering (81), Microbiology (92), Synthetic Biology (90.5)

(2)RESEARCH EXPERIENCE

Since the project1 and project 2 have not yet been completed and published, the responsible content and main responsibilities will be presented in the detailed conversation with the teacher

Project 1: Characterization and Phylogenetic Analysis of optrA-Positive Linezolid-Resistant MRSA Causing Bloodstream Infections in China

Project 2: Staphylococcus aureus Research Project at Hanzhong 3201 Hospital

Project 3: Eucalyptus Germplasm Innovation and Industrialization Development
Core Responsibilities:
   - Led the development of a molecular fingerprint database for 50 eucalyptus varieties using Python+Django, MySQL, and HTML/CSS/JavaScript, enabling data interaction and front-end visualization.
   - Integrated and reanalyzed multi-omics data (RNA-seq, resequencing, T2T genome), developing functional modules for sequence alignment, data query, and bioinformatics analysis to support germplasm research.

Project 4: Genome-Wide Characterization and Gene Expression Analysis of PHR Transcription Factors in Eucalyptus
Core Responsibilities:
  - Assisted in project application and integrated abiotic stress transcriptome data of multiple eucalyptus species.
  - Conducted upstream data analysis and followed up on subsequent multi-omics research work.

(3)PUBLICATIONS
[1] In Preparation: Characteristics and Phylogenetic Analysis of optrA-Positive Linezolid-Resistant MRSA Causing Bloodstream Infections in China (Planned for CAS Tier 1 Journal; Co-First Author)
[2] Preprint: Unveiling the PHR-centered regulatory network orchestrating the phosphate starvation signaling in Chinese fir (Cunninghamia lanceolata) (IF=5.75, Plant Journal, JCR Q1, CAS Tier 1; Co-First Author)
[3] Published: Genome-Wide Identification and Gene Expression Analysis of the Malate Dehydrogenase (MDH) Gene Family in Eucalyptus grandis (IF=4.8, Frontiers in Plant Science, JCR Q1, CAS Tier 2; First Author)
[4] Published: Xu, H.; Xing, Y.; Li, G.; et al. Decoding PHR-Orchestrated Stress Adaptation: A Genome-Wide Integrative Analysis of Transcriptional Regulation Under Abiotic Stress in Eucalyptus grandis. Int. J. Mol. Sci. 2025, 26, 2958. https://doi.org/10.3390/ijms26072958 (IF=4.9, JCR Q1, CAS Tier 3; Second Author)

(4)TECHNICAL SKILLS
Experimental Skills
    - Proficient in gene cloning, DNA/RNA extraction, Arabidopsis protoplast transformation, quantitative real-time PCR (qPCR), plant tissue culture, PAT-seq library construction, Western blotting, and tobacco infiltration experiments.
Analytical Skills
     - Programming Languages: Skilled in R, Python, and Linux command line.
    - Omics Analysis: Proficient in RNA-seq, DAP-seq, epitranscriptomics (m6anet pipeline), and second-generation resequencing analysis; experienced in GWAS, single-cell transcriptome analysis, T2T genome assembly, bacterial genome annotation, and pan-genomics analysis.
   - Analysis Tools: Mastered Beast (temporal phylogenetic tree construction), scientific plotting software (Origin, GraphPad Prism, Cytoscape, Adobe Illustrator, TBtools), and downstream omics data analysis tools, SCI figure preparation software, and online analysis platforms.

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