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[求助]
求理解一段话谢谢
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| A standard β-sitosterol sample was procured from Fluka Chemicals and analyzed at the laboratory by gas chromatography (GC). Fish oil (Mega-Shelcal capsules from Elder Pharmaceuticals, India) was used as the source of eicosapentanoic acid (EPA) and docosahexaenoic acid (DHA), and the GC analysis of the fish oil showed that the oil contained 32 % EPA and 22 % DHA. Refined, bleached flaxseed oil procured from V.K.V.K. Oil Limited, Kolkata, India, was used as the source of alpha linolenic acid (ALA), and the GC analysis of the oil showed the presence of 54 % ALA in the oil. Thermomyces lanuginosus lipase (Lipozyme TLIM) used as biocatalyst, was a generous gift from Novozyme India, Ltd., Bangalore, India. Phytosterol esters were formed by enzymatic transesterification reactions in a packed bed reactor and their fatty acid compositions were analyzed by GC (Sengupta et al. 2010). The yield of phytosterol esters were 95 % EPA-DHA rich sterol ester and 95.2 % ALA rich sterol ester. The sterol esters were purified using column chromatography. |
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2楼2015-08-03 15:29:02
3楼2015-08-04 09:22:32
baoshanqiu
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标准β-谷甾醇样品从Fluka化学试剂公司获得，实验室用气相色谱法（GC）进行分析。鱼油（来自于印度Elder药剂公司的巨型Shelcal 胶囊）作为二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的来源，用GC分析鱼油表明该油含32%EPA和22%DHA。精炼、漂白的亚麻籽油从印度加尔各答VKVK油料有限公司获得，用作α-亚麻酸(ALA)的来源，GC分析表明该油含54%ALA。疏棉状嗜热丝孢菌酯酶（酯酶 TLIM)作为生物催化剂，由印度班加罗尔Novozyme India, Ltd慷慨惠赠。植物甾醇酯是在填料床反应器内用酶转酯反应合成，其脂肪酸组成用GC分析（Sengupta 等. 2010)。植物甾醇酯的得率为95%富含EPA-DHA甾醇脂和95.2%富含ALA甾醇脂。这些甾醇脂用柱色谱纯化。 从这段文字看，没有使用EPA-DHA phytosterol ester，而是合成的phytosterol ester富含EPA-DHA，也没说具体含多少。从原文结果表1中可见C20：5含量为39.20+-1.10%，C22：6含量为31.98+-1.63%。 |
4楼2015-08-04 23:01:59
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