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[求助] boronate buffer (pH 7.8, 100 mM)配制

One gram of Bovine serum albumin and 10 mg of fluorescein isothiocyanate were dissolved into 100 mL boronate buffer (pH 7.8, 100 mM). Then the solution was stirred at 22℃ for overnight, followed by the purification by gel filtration. Purity of the fluorescein-labeled bovine serum albumin was checked by HPLC, followed by the lyophilization.
求教下这个怎么操作?需要注意什么?( boronate buffer (pH 7.8, 100 mM).这个没配制过)
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