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| Hongtao Nie, Hongbo Niu, Liqiang Zhao, Feng Yang, Xiwu Yan*, Guofan Zhang. 2015. Genetic diversity and structure of Manila clam (Ruditapes philippinarum) populations from Liaodong peninsula revealed by SSR markers. Biochemical Systematics and Ecology 59: 116-125. (SCI) |
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Ö±½Ó¸´ÖƵÄÏêϸ¼ìË÷ÐÅÏ¢ ¹§Ï²¹§Ï²![]() Genetic diversity and structure of Manila clam (Ruditapes philippinarum) populations from Liaodong peninsula revealed by SSR markers ×÷Õß:Nie, HT (Nie, Hongtao)[ 1,2 ] ; Niu, HB (Niu, Hongbo)[ 1 ] ; Zhao, LQ (Zhao, Liqiang)[ 1 ] ; Yang, F (Yang, Feng)[ 1 ] ; Yan, XW (Yan, Xiwu)[ 1 ] ; Zhang, GF (Zhang, Guofan)[ 3 ] BIOCHEMICAL SYSTEMATICS AND ECOLOGY ¾í: 59 Ò³: 116-125 DOI: 10.1016/j.bse.2014.12.029 ³ö°æÄê: APR 2015 ²é¿´ÆÚ¿¯ÐÅÏ¢ ÕªÒª Manila clam (Ruditapes philippinarum) is an important commercially marine bivalve, and its wild populations have been severely declining in the coast of China during the last decade. In this study, a set of 7 genomic simple sequence repeat (SSR), and 5 expressed sequences tag (EST)-derived SSR markers were analyzed on eight wild R. philippinarum populations to assess the genetic diversity and population differentiation. A total of 114 alleles were detected on 12 loci, and the number of alleles per locus in each population ranged from 2 to 11, and allelic richness per locus varied from 2.00 to 10.88 for each sample. The average of observed and expected heterozygosity ranged from 0.386 to 0.550, and from 0.533 to 0.707, respectively. Pairwise F-ST values indicated that all population pairs had significant genetic differentiation (overall F-ST = 0.242, P < 0.01). Cluster analysis using unweighted pair group method with the arithmetic mean (UPGMA) separated the eight populations into two groups. This study will shed light on the domestication and cultivation on population genetic diversity of R. philippinarum, and also provide the foundation for conservation of R. philippinarum germplasm resources in clam breeding practices. (C) 2015 Elsevier Ltd. All rights reserved. ¹Ø¼ü´Ê ×÷Õ߹ؼü´Ê:Ruditapes philippinarum; Microsatellite; Genetic diversity; Genetic differentiation; Conservation KeyWords Plus:CROSS-SPECIES AMPLIFICATION; MICROSATELLITE DNA; CRASSOSTREA-GIGAS; PACIFIC OYSTER; MARINE; DIFFERENTIATION; PHYLOGEOGRAPHY; CONSERVATION; VARIABILITY; SOFTWARE ×÷ÕßÐÅÏ¢ ͨѶ×÷ÕßµØÖ·: Yan, XW (ͨѶ×÷Õß) ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ Dalian Ocean Univ, Engn & Technol Res Ctr Shellfish Breeding Liaonin, Dalian 116023, Peoples R China. µØÖ·: ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ [ 1 ] Dalian Ocean Univ, Engn & Technol Res Ctr Shellfish Breeding Liaonin, Dalian 116023, Peoples R China ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ [ 2 ] Dalian Ocean Univ, Minist Agr, Key Lab Mariculture & Stock Enhancement North Chi, Dalian 116023, Peoples R China ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ [ 3 ] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China µç×ÓÓʼþµØÖ·:yanxiwu@dlou.edu.cn »ù½ð×ÊÖúÖÂл »ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ National Natural Science Foundation of China 31302183 Program for Liaoning Excellent Talents in University LJQ2014076 Cultivation Plan for Youth Agricultural Science and Technology Innovative Talents of Liaoning Province 2014004 Modern Agro-industry Technology Research System CARS-48 National Basic Research Program of China, 973 Program 2010CB126402 National High Technology Research and Development Program, 863 Program 2012AA10A410-2 ²é¿´»ù½ð×ÊÖúÐÅÏ¢ ³ö°æÉÌ PERGAMON-ELSEVIER SCIENCE LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND Àà±ð / ·ÖÀà Ñо¿·½Ïò:Biochemistry & Molecular Biology; Environmental Sciences & Ecology; Evolutionary Biology Web of Science Àà±ð:Biochemistry & Molecular Biology; Ecology; Evolutionary Biology ÎÄÏ×ÐÅÏ¢ ÎÄÏ×ÀàÐÍ:Article ÓïÖÖ:English Èë²ØºÅ: WOS:000353753500018 ISSN: 0305-1978 eISSN: 1873-2925 ÆÚ¿¯ÐÅÏ¢ Impact Factor (Ó°ÏìÒò×Ó): Journal Citation Reports® ÆäËûÐÅÏ¢ IDS ºÅ: CH1AM Web of Science ºËÐĺϼ¯ÖÐµÄ "ÒýÓõIJο¼ÎÄÏ×": 51 Web of Science ºËÐĺϼ¯ÖÐµÄ "±»ÒýƵ´Î": 0 |

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