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Enhancement of l-phenylalanine production by engineered Escherichia coli using phased exponential l-tyrosine feeding combined with nitrogen source optimization. ×÷Õß:Yuan, Peipei; Cao, Weijia; Wang, Zhen; Chen, Kequan; Li, Yan; Ouyang, Pingkai Journal of bioscience and bioengineering ¾í:120 ÆÚ:1 Ò³:36-40 DOI:10.1016/j.jbiosc.2014.12.002 ³ö°æÄê:2015-Jul (Epub 2014 Dec 29) ÕªÒª Nitrogen source optimization combined with phased exponential l-tyrosine feeding was employed to enhance l-phenylalanine production by a tyrosine-auxotroph strain, Escherichia coli YP1617. The absence of (NH4)2SO4, the use of corn steep powder and yeast extract as composite organic nitrogen source were more suitable for cell growth and l-phenylalanine production. Moreover, the optimal initial l-tyrosine level was 0.3gL(-1) and exponential l-tyrosine feeding slightly improved l-phenylalanine production. Nerveless, l-phenylalanine production was greatly enhanced by a strategy of phased exponential l-tyrosine feeding, where exponential feeding was started at the set specific growth rate of 0.08, 0.05, and 0.02h(-1) after 12, 32, and 52h, respectively. Compared with exponential l-tyrosine feeding at the set specific growth rate of 0.08h(-1), the developed strategy obtained a 15.33% increase in l-phenylalanine production (l-phenylalanine of 56.20gL(-1)) and a 45.28% decrease in l-tyrosine supplementation. Copyright 2014 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved. ¹Ø¼ü´Ê ¹Ø¼ü´ÊÁбí:Engineered Escherichia coli; Fed-batch; Nitrogen source; l-Phenylalanine; l-Tyrosine feeding ×÷ÕßÐÅÏ¢ µØÖ·:State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211816, People's Republic of China. ÎÄÏ×ÐÅÏ¢ ÎÄÏ×ÀàÐÍ:Journal Article ÓïÖÖ:English PubMed ID:25553973 NLM Ψһ ID:100888800 ´´½¨ÈÕÆÚ: 05 Jun 2015 µç×Ó³ö°æ:29 Dec 2014 ¹ú¼Ò/µØÇø: Japan ISSN:1347-4421 ÆäËûÐÅÏ¢ ·ÖÖ÷Ìâ×Ó¿â:Index Medicus ״̬:In-Data-Review ûÓб»ºËÐļ¯ÊÕ¼£¬Ã»ÓмìË÷ºÅ |

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