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关于杜氏盐藻的翻译
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翻译下这个摘要Evaluation of total reducing capacity in three Dunaliella salina (Dunal) Teodoresco isolates Abstract To evaluate reducing capacity of the halophilic green alga Dunaliella salina, total reducing substances together with total carotenoids were extracted from three D. salina isolates at both the logarithmic and the stationary growth phases. Extractions were performed using hexane (H), ethyl acetate (EA), methanol (M), and water (W). Total reducing capacities of the extracts were determined by ferric ion reducing antioxidant power (FRAP), 2, 2-diphenyl-1-picrylhydrazyl (DPPH), and Folin-Cioculteu (F-C) assay methods. As the best solvent, ethyl acetate was more efficient in extracting total carotenoids and total reducing substances, as is evident by the higher total reducing capacities measured by the FRAP, the DPPH, and the F-C assays. Isolate difference in reducing capacity was noticeable in the isolate MSI-2 with significantly higher extractable total reducing substances and total carotenoids. In the stationary growth phase, total reducing capacity was higher compared with the logarithmic growth phase; in particular, with water as the solvent, fivefold increase in total reducing capacity was observed. As a result, solvent extracting efficiency changed from EA>H, M, W at the logarithmic phase to EA>W>H, M at the stationary phase with the F-C assay, and from EA>H>M>W to EA>W>M>H (P<0.05) when the FRAP and the DPPH assays were used. Patterns of changes in total reducing capacity were similar in the three assays with highest correlations of 0.940 and 0.916 at P<0.05 between the F-C and the FRAP assays at the logarithmic and at the stationary growth phases, respectively. Weakest correlation (R2=0.518, P<0.05) was observed between total carotenoids and the DPPH assay at the stationary growth phase. It is concluded that D. salina not only is a good source of β-carotene, but also, its reducing substances may contribute to the antioxidant capacity of this microalga。 急求感谢 |
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