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【答案】应助回帖
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peterzc: 金币+10, 翻译EPI+1 2015-06-05 18:20:16
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Based on previous studies, a water-soluble CdSe quantum dots with narrow particle size distribution and high fluorescence quantum yield were synthesized by a one-step reduction method from selenium oxide and hydrazine hydrate. On the basis of this result, the quantum dots of CdSs were synthesized and used as the fluorescence source to construct a quantum dot-base molecular switch. The alizarin red can effectively quench the fluorescence of quantum dots, and the fact that alizarin red can accept the lone pair electrons on the amino nitrogen to form a n- pai coordination complex, therefore, adding an amino-containing Amikacin to the CdSe quantum dot-alizarin red fluorescence quenching system can restore the fluorescence from quantum dots. Based this we establishes a new fluorescence recovery method for the quantitative measurement of Amikacin; Dithizone binds to the surface of quantum dots, it can also cause quenching of fluorescence from quantum dots through fluorescence resonance energy transfer. There is a strong coordination between mercury ion and dithizone, and therefore, with the addition of mercury ions to dithizone-modified CdSe quantum dots, the dithizone was stripped from the surface of quantum dots, which resulted in the restoration of the fluorescence from quantum dots. Based this, another new fluorescence recovery method for the measurement of Hg2+ was established. The research of this thesis provided a significant advancement for the application of CdSe quantum dots, and it also provides new methods for the determination of Amikacin and mercury ion. |
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