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shaishaisong

木虫 (正式写手)

[求助] 请教各位大神如何从秀丽隐杆线虫中提取脂质 已有1人参与

请教各位大神如何从秀丽隐杆线虫中提取脂质
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water128

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4楼2015-08-26 14:08:36
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广之

金虫 (正式写手)

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可以读读这篇protocol:
Isolating lipid droplets from multiple species
http://www.nature.com/nprot/journal/v8/n1/abs/nprot.2012.142.html
2楼2015-06-25 13:50:04
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广之

金虫 (正式写手)

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2楼: Originally posted by 广之 at 2015-06-25 13:50:04
可以读读这篇protocol:
Isolating lipid droplets from multiple species
http://www.nature.com/nprot/journal/v8/n1/abs/nprot.2012.142.html

The Caenorhabditis elegans lipidome: A primer for lipid analysis in Caenorhabditis elegans
http://www.sciencedirect.com/sci ... i/S0003986115002672

C. elegans Major Fats Are Stored in Vesicles Distinct from Lysosome-Related Organelles
http://www.sciencedirect.com/sci ... i/S1550413109003015
3楼2015-06-25 14:01:56
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广之

金虫 (正式写手)

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4楼: Originally posted by water128 at 2015-08-26 14:08:36
请教您一下,它那个里面4×10^5 worms,究竟是怎么界定出来的?我怎么对虫子数目进行一个估算呢?...

"To count worms - just dilute your sample and then count the number using a stereo microscope and then extrapolate the number of your original sample." For example, if you counted 100 larvae in 1 μl and have 10 ml of larvae, then you have a total of 100 × 10,000 =1,000,000 larvae.
可以参看下面的protocols
High-throughput fluorescence-based isolation of live C. elegans larvae
http://www.nature.com/nprot/journal/v7/n8/abs/nprot.2012.084.html
5楼2015-08-26 18:01:02
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