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1. The authors could consider other parameters for studying the affinity of the enzyme for the substrate. They did the comparation with the free enzyme and immobilized enzyme for one substrate. The Michaeliana Kinetic is valid specially when the comparation is between different enzymes in front of one substrate. ±¾È˵ĿÎÌâÊôÓÚ½»²æÑ§¿ÆµÄ£¬¶ÔøÕâ·½Ãæ²»ÊÇÌØ±ðµÄÊ죬ÎÒ֮ǰ¿´µÄÎÄÏ×Àï±í´ïøºÍµ×ÎïµÄaffinityµÄÓõͼÊÇÃ×Êϳ£Êý°¡£¬Éó¸åÈËÈÃÎÒ¿¼ÂÇÓÃÆäËûParametersÀ´Ñо¿Ã¸ºÍµ×ÎïµÄÇ׺ÍÁ¦£¬ÎÒ¶¼²»ÖªµÀ»¹ÓÐʲô£¬Çë´óÉñÖ¸µãÒ»ÏÂ~ -The biocatalyst immobilized can present diffusion effects. Then, the authors did not prove that the km value is really different on the figure 3. -Catalytic efficiency decreased after the immobilization process but the km value must be done for each biocatalyst. ʣϵÄÕâÁ½ÌõÎÒ²»Ì«Ã÷°×Éó¸åÈ˵ÄÒâ˼£¬ÇóÖ¸µã°¡ÇóÖ¸µã£¡£¡£¡ |
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