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irena59

银虫 (初入文坛)

[交流] 急求RNASIN inhibitor 基因序列 2008-10-1

急求RNASIN inhibitor 基因序列,恳请各位帮忙!

[ Last edited by irena59 on 2008-7-17 at 11:28 ]
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香菱儿

铁杆木虫 (著名写手)


irena59(金币+1,VIP+0): 4-22 13:48
RNASIN是什么生物啊?
觉得应该是 RNase inhibitor 吧?您是要找 RNase inhibitor 的基因序列吗?
漫洒英雄泪,相离处士家。谢慈悲剃度在莲台下。没缘法转眼分离乍。赤条条来去无牵挂。那里讨烟蓑雨笠卷单行?一任俺芒鞋破钵随缘化!
2楼2008-07-17 10:25:47
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irena59

银虫 (初入文坛)

是的,不好意思,见笑了,不知道您能不能帮忙?
3楼2008-07-17 11:18:38
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yxwjimmy

木虫 (正式写手)


irena59(金币+1,VIP+0): 4-22 13:48
RNase Inhibitor is a recombinant human placental protein which specifically inhibits ribonucleases (RNases) A, B and C (1). It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).

The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 1014 (2).

Source:
An E. coli strain that carries the Ribonuclease Inhibitor gene from human placenta.
4楼2008-07-17 13:10:13
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yxwjimmy

木虫 (正式写手)

★ ★ ★ ★ ★ ★
irena59(金币+5,VIP+0):think you
irena59(金币+1,VIP+0): 4-22 13:48
Search it in NCBI, and you can get some information on it.
5楼2008-07-17 13:11:33
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irena59

银虫 (初入文坛)

Think your suggestion! I have done a  lot in ncbi
but got noting about it ,Maybe the article from neb can help. but how can i get this one . GOOGLE is unavaible!
6楼2008-07-17 15:06:22
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