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irena59

Òø³æ (³õÈëÎÄ̳)

[½»Á÷] ¼±ÇóRNASIN inhibitor »ùÒòÐòÁÐ 2008-10-1

¼±ÇóRNASIN inhibitor »ùÒòÐòÁУ¬¿ÒÇë¸÷λ°ï棡

[ Last edited by irena59 on 2008-7-17 at 11:28 ]
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Ìú¸Ëľ³æ (ÖøÃûдÊÖ)

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irena59(½ð±Ò+1,VIP+0): 4-22 13:48
RNASINÊÇʲôÉúÎï°¡£¿
¾õµÃÓ¦¸ÃÊÇ RNase inhibitor °É£¿ÄúÊÇÒªÕÒ RNase inhibitor µÄ»ùÒòÐòÁÐÂð£¿
ÂþÈ÷Ó¢ÐÛÀᣬÏàÀ봦ʿ¼Ò¡£Ð»´È±¯Ìê¶ÈÔÚÁ«Ì¨Ï¡£Ã»Ôµ·¨×ªÑÛ·ÖÀëÕ§¡£³àÌõÌõÀ´È¥ÎÞÇ£¹Ò¡£ÄÇÀïÌÖÑÌËòÓêóÒ¾íµ¥ÐУ¿Ò»Èΰ³Ã¢Ð¬ÆÆ²§ËæÔµ»¯£¡
2Â¥2008-07-17 10:25:47
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irena59

Òø³æ (³õÈëÎÄ̳)

Êǵ쬲»ºÃÒâ˼£¬¼ûЦÁË£¬²»ÖªµÀÄúÄܲ»Äܰïæ£¿
3Â¥2008-07-17 11:18:38
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

yxwjimmy

ľ³æ (ÕýʽдÊÖ)

¡ï
irena59(½ð±Ò+1,VIP+0): 4-22 13:48
RNase Inhibitor is a recombinant human placental protein which specifically inhibits ribonucleases (RNases) A, B and C (1). It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3).

The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 1014 (2).

Source:
An E. coli strain that carries the Ribonuclease Inhibitor gene from human placenta.
4Â¥2008-07-17 13:10:13
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

yxwjimmy

ľ³æ (ÕýʽдÊÖ)

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irena59(½ð±Ò+5,VIP+0):think you
irena59(½ð±Ò+1,VIP+0): 4-22 13:48
Search it in NCBI, and you can get some information on it.
5Â¥2008-07-17 13:11:33
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

irena59

Òø³æ (³õÈëÎÄ̳)

Think your suggestion! I have done a  lot in ncbi
but got noting about it ,Maybe the article from neb can help. but how can i get this one . GOOGLE is unavaible!
6Â¥2008-07-17 15:06:22
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
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