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Total sugar Soluble sugar was determined by high performance liq-uid chromatography (Shimadzu, LC-10A) equipped with NH2P-50 column (4.6 mm diam. ¡Á 250 mm) and RI detector.11 The column was packed with SUS 316. The mobile phase was acetonitrile: water = 75:25 at a flow rate of 1.0 ml min−1 and injection volume is 20 l as described by Ajlouni et al.12 A 20-g sample from ten ginseng roots was homogenized with 20 ml of 75% acetonitrile. The homogenate was centrifuged at 12 000 ¡Á g for 20 min. The supernatant was taken as sample to measure the sugar content. Standard solu-tions of fructose, glucose and sucrose were employed at a concentration of 5 mg ml−1. Final results were expressed as mg g−1 fresh weight. |
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- ·ÒëEPI: 68
- Ó¦Öú: 135 (¸ßÖÐÉú)
- ¹ó±ö: 1.097
- ½ð±Ò: 14467.1
- É¢½ð: 3921
- ºì»¨: 102
- ɳ·¢: 3
- Ìû×Ó: 2121
- ÔÚÏß: 396.8Сʱ
- ³æºÅ: 1783324
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3Â¥2015-05-15 14:12:47
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ÖÁ×ðľ³æ (ÖøÃûдÊÖ)
- ·ÒëEPI: 68
- Ó¦Öú: 135 (¸ßÖÐÉú)
- ¹ó±ö: 1.097
- ½ð±Ò: 14467.1
- É¢½ð: 3921
- ºì»¨: 102
- ɳ·¢: 3
- Ìû×Ó: 2121
- ÔÚÏß: 396.8Сʱ
- ³æºÅ: 1783324
- ×¢²á: 2012-04-27
- ÐÔ±ð: GG
- רҵ: »·¾³¹¤³Ì
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yi8yi25: ½ð±Ò+5, ·ÒëEPI+1, ¡ïÓаïÖú 2015-05-15 14:42:02
yi8yi25: ½ð±Ò+5, ·ÒëEPI+1, ¡ïÓаïÖú 2015-05-15 14:42:02
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Total sugar ×ÜÌÇ Soluble sugar was determined by high performance liquid chromatography (Shimadzu, LC-10A) equipped with NH2P-50 column (4.6 mm diam. ¡Á 250 mm) and RI detector. The column was packed with SUS 316¡£ ÓÃ×°±¸ÓÐNH2P-50ÐÍÉ«Æ×Öù£¨4.6 mm Ö±¾¶ ¡Á 250 mm) £©¼°RI¼ì²âÆ÷µÄ¸ßЧҺÏàÉ«Æ×£¨ÈÕ±¾µº½ò¹«Ë¾£¬ LC-10A£©À´²â¶¨¿ÉÈÜÐÔÌÇ¡£ÓÃ316ÐͲ»Ðâ¸ÖÌî³äÖùÌå¡£ The mobile phase was acetonitrile: water = 75:25 at a flow rate of 1.0 ml min−1 and injection volume is 20 l as described by Ajlouni et al. ¸ù¾Ý AjlouniµÈÈËËùÊö£¬Á÷¶¯ÏàÊÇÁ÷Á¿Îª 1.0 ml min−1µÄÒÒëæ:Ë®= 75:25 »ìºÏÒº£¬½øÑùÁ¿Îª 20 l ¡£ A 20-g sample from ten ginseng roots was homogenized with 20 ml of 75% acetonitrile. The homogenate was centrifuged at 12 000 ¡Á g for 20 min. The supernatant was taken as sample to measure the sugar content. 20g´ÓÈ˲θùÖлñµÃµÄÑùÆ·Óë20ml 75% ÒÒëæ»ìºÏ¾ùÔÈ£¬È»ºóÔÚ 12 000 ¡Á gÌõ¼þÏÂÀëÐÄ 20 min¡£È¡ÉϲãÇåÒº×÷ΪÑùÆ·À´µÄ²â¶¨ÌǺ¬Á¿¡£ Standard solutions of fructose, glucose and sucrose were employed at a concentration of 5 mg ml−1. Final results were expressed as mg g−1 fresh weight. ʹÓõĹûÌÇ¡¢ÆÏÌÑÌÇÒÔ¼°ÕáÌDZê×¼ÈÜҺŨ¶È¶¼Îª 5 mg ml−1¡£×îÖÕ£¬½á¹ûÓÃÏÊÖØ mg g−1 À´±íʾ¡£ |

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