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yi8yi25

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Total sugar

Soluble sugar was determined by high performance liq-uid chromatography (Shimadzu, LC-10A) equipped with NH2P-50 column (4.6 mm diam. × 250 mm) and RI detector.11 The column was packed with SUS 316. The mobile phase was acetonitrile: water = 75:25 at a flow rate of 1.0 ml min−1 and injection volume is 20 l as described by Ajlouni et al.12 A 20-g sample from ten ginseng roots was homogenized with 20 ml of 75% acetonitrile. The homogenate was centrifuged at 12 000 × g for 20 min. The supernatant was taken as sample to measure the sugar content. Standard solu-tions of fructose, glucose and sucrose were employed at a concentration of 5 mg ml−1. Final results were expressed as mg g−1 fresh weight.

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灿灿妹

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yi8yi25: 金币+5, 翻译EPI+1, ★有帮助 2015-05-15 14:42:02

Total sugar
总糖
Soluble sugar was determined by high performance liquid chromatography (Shimadzu, LC-10A) equipped with NH2P-50 column (4.6 mm diam. × 250 mm) and RI detector. The column was packed with SUS 316。
用装备有NH2P-50型色谱柱（4.6 mm 直径 × 250 mm) ）及RI检测器的高效液相色谱（日本岛津公司， LC-10A）来测定可溶性糖。用316型不锈钢填充柱体。
The mobile phase was acetonitrile: water = 75:25 at a flow rate of 1.0 ml min−1 and injection volume is 20 l as described by Ajlouni et al.
根据 Ajlouni等人所述，流动相是流量为 1.0 ml min−1的乙腈:水= 75:25 混合液，进样量为 20 l 。
A 20-g sample from ten ginseng roots was homogenized with 20 ml of 75% acetonitrile. The homogenate was centrifuged at 12 000 × g for 20 min. The supernatant was taken as sample to measure the sugar content.
20g从人参根中获得的样品与20ml 75% 乙腈混合均匀，然后在 12 000 × g条件下离心 20 min。取上层清液作为样品来的测定糖含量。
Standard solutions of fructose, glucose and sucrose were employed at a concentration of 5 mg ml−1. Final results were expressed as mg g−1 fresh weight.
使用的果糖、葡萄糖以及蔗糖标准溶液浓度都为 5 mg ml−1。最终，结果用鲜重 mg g−1 来表示。