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¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï ¡ï °®ÓëÓêÏÂ: ½ð±Ò+1 2015-05-06 20:54:26 qinsmoon0110: ½ð±Ò+20, ·ÒëEPI+1, ¡ï¡ï¡ï¡ï¡ï×î¼Ñ´ð°¸, ºÜÔÞ°¦£¬ÄǸö¼ýÍ·±íʾµÄ¶«¶«²»Ó÷Òë³öÀ´Âð 2015-05-07 10:59:50
The glycosidic linkages of LSPs I and II were determined by methylation method and analyzed by GC/MS.
Óü׻ù»¯·¨È·¶¨ÁË LSPs I ºÍ II ÖеÄÌÇÜÕ¼ü£¬²¢Óà GC/MS¶ÔÆä½øÐзÖÎö¡£
As shown in Table 4, the glycosidic linkages of LSP I were in coincidence with those of LSP II.
Èç±í4Ëùʾ£¬ LSP I ÖеÄÌÇÜÕ¼üÓë LSP IIÖеÄÏà·û¡£
¡ú6)-Glc-(1¡úwas detected in both LSPs I and II. However, ¡ú2,4)-Glc terminal was only found in LSP I.
¡ú6)-Glc-(1¡ú ÔÚÁ½Õ߼䶼¼ì²âµ½ÁË£¬¶ø ¡ú2,4)-GlcÖ»ÔÚ LSP IÖз¢ÏÖÁË¡£
Galactose had two linking forms,which were¡ú3)-Gal-(1¡úand¡ú6)-Gal-(1¡ú, respectively.
°ëÈéÌǾßÓÐÁ½ÖÖÁ¬½Ó·½Ê½£¬·Ö±ðÊÇ¡ú3)-Gal-(1¡ú ºÍ¡ú6)-Gal-(1¡ú¡£
LSP I ºÍII ÖиʶÌǵÄÌÇÜÕ¼ü´æÔÚÏÔÖøÐÔ²îÒì¡£
The glycosidic linkages of Mannose in LSP I and II were significantly different. LSP I had¡ú2)-Man-(1¡úand¡ú3,6-Man-(1¡ú, whereas LSP II had¡ú2)-Man-(1¡úand¡ú3)-Man-(1¡ú.
LSP I ºÍII ÖиʶÌǵÄÌÇÜÕ¼ü´æÔÚÏÔÖøÐÔ²îÒì¡£ LSP I¾ßÓСú2)-Man-(1¡úand¡ú3,6-Man-(1¡ú£¬¶ø LSP II ¾ßÓСú2)-Man-(1¡úand¡ú3)-Man-(1¡ú¡£
Arabinose and fructose were found to be linked as¡ú5)-Ara-(1¡úand¡ú3)-Fru-(1¡ú.
Ê÷½ºÈ©õ±ºÍ¹ûÌÇÖж¼·¢ÏÖ´æÔÚ¡ú5)-Ara-(1¡úand¡ú3)-Fru-(1¡úµÄÁ¬½Ó·½Ê½¡£
Furthermore, the rela-tive molar percentage of each linkage was different between LSPs I and II. ¡ú3)-Gal-(1¡úhad a higher relative molar percentage than¡ú6)-Gal-(1¡úfor LSP I. Nevertheless, an opposite result was obtained for LSP II.
´ËÍ⣬ LSPs I ºÍ IIÁ½Õß¼ä¸÷Á¬½Ó·½Ê½µÄÏà¶ÔĦ¶û°Ù·Ö±ÈÒ²²»Í¬¡£¶ÔÓÚ LSP I£¬¡ú3)-Gal-(1¡ú±È¡ú6)-Gal-(1¡úÓиü¸ßµÄÏà¶ÔĦ¶û°Ù·Ö±È£»¶ø¶ÔÓÚ LSP II£¬½á¹ûÇ¡ºÃÏà·´¡£ |
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