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Substrate micronization So-called “wet” disintegration of steroid particles based on ultrasonic wave in combination with detergents can facilitate the contact between substrate and whole-cell biocatalyst. Organic-aqueous biphasic systems Two-phase organic system based on n-hexane,butanol, benzene etc. may increase the productivity of biotransformation due to dissolution of steroid substrate/product and make easier downstream processing. Major disadvantages concerned inactivation or destruction of biocatalyst in the apolar phase. Water-miscible organic co-solvents Water-miscible organic solvents are used to overcome the low solubility of steroid substrates/products and hence intensify the productivity. Cloud-point systems based on nonionic surfactant This approach was regarded as a novel two-phase partitioning system and used for solubilization and phase separation in whole cell conversion. It enables elimination of substrate and product inhibition, and prevents further degradation of product. Liquid polymer based systems Liquid polymers as components of the bioconversion media (silicon oil, polypropylene glycol, polydimethyl siloxane, etc.) are used for solubilization of sterol compounds and acceleration of steroid accumulation. |
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| 底物微粉化 所谓基于与清洁剂组合超声波的甾类化合物粒子的"湿品"粉碎能够促进底物与全细胞生物催化剂的接触。有机-水双相系统 基于正己烷、丁醇、苯等的二相有机系统可能由于甾类化合物底物／产物的溶解而提高生物转化生产力并使下游处理更容易一些。 主要缺点关联到非极性相中生物催化剂的失活或损坏。水混溶性有机共溶剂 水混溶性有机溶剂用于克服甾类底物／产物的低溶解度问题，从而提高生产力。基于非离子表面活性剂的浊点系统 这种方法被认为是一种新颖的两相分配系统，用于全细胞转换的溶解和相分离。 这能够消除底物和产物抑制作用并防止产物的进一步降解。液态聚合物系统 作为生物转化培养基（硅油、聚丙二醇、聚二甲基硅氧烷,等等)组分的液态聚合物用于甾醇类化合物的溶解和加速甾类积累。 |
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