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qian86812

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[求助] 微藻多样性分析要不要翻译成氨基酸进行分析

投稿杂志,其中review 说多样性的分析不能把核酸翻译成氨基酸进行多样性的分析,这样会错过很多信息。

Reviewer 1: re-analysis of data using nucleotide instead of amino-acid sequences. - I agree with the reviewer that nucleotide data should be used. There is not only the problem with the phylogeny but also with the identity of OTUs. A majority of interpretations comment ecology of OTU identified to species, but algal species could be hardly inferred from amino acid data. In diatoms, two partial rbcL sequences that differ in one nucleotide can represent both one and two species.

还有在NCBI库中blast的时候勾选去掉了uncultured /environmental samples sequences  这样对不对?还有怎么进行uncultured samples sequences 的定义?

急急急!!!求指导
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