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[求助]
几句论文求大神翻译成中文 谢谢
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2.5. Acridine Orange Staining. At the fifth day of culturing, cells grown on different filmswerewashedwith PBS and stained with 0.1mg/mL acridine orange (Sigma-Aldrich) at room temperature in darkness for 30 min. Then, all the samples were washed with PBS to remove the residual dye and observed through an inverted fluorescencemicroscope (Nikon Ti). 2.6. Cell Viability Assay. Cell viability was measured with Cell Counting Kit-8 (CCK-8, Dojindo, Japan) according to the manufacture’s protocol. The amount of the formazan dye generated by the activity of dehydrogenases in cells on highly water-soluble tetrazolium salt is directly proportional to the number of living cells. Cells were cultured on different PHBVfilms in 48-well plates with six repeats. In brief, at 1, 3, and 5 days after cell seeding, 20 μL ofCCK-8 solution was added to each well and incubated in the incubator for 3 h; then, 100 μL of solution per well was transferred to a 96-well plate and read at 450 nm with a microplate reader (Symergy HT, Biotek). Cell viability can be continually tested in the same well without any toxicity to the cells. |
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