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几句论文求大神翻译成中文 谢谢
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2.7. Serum Adsorption Tests by Optical Waveguide Lightmode Spectroscopy (OWLS). Nonspecific protein adsorption from serum was tested on unmodified and various modified PHBV films by OWLS.30,33 In brief, 2 wt % of PHBV was spin-coated onto waveguide chips, and subsequent surface modifications were carried out as described in Section 2.1. For comparison with the properties of MAL-PEG-grafted PHBV films, CH3OPEG-grafted PHBV films (PHBV-PEGOCH3) were prepared by the reaction of NH3-plasma-treated PHBV with 2 mM N-hydroxysuccinimidyl ester of methoxypoly-(ethylene glycol) propionic acid (mol wt 2 or 5 kDa, NHS-PEG-OCH3, Jenkem Technology Co. Ltd., Beijing, China) in HEPES-NaCl buffer (10 mM HEPES, 150 mM NaCl, pH 7.2) for 1 h. |
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jiangguofeng
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Malyhon: 金币+8, 翻译EPI+1, ★★★★★最佳答案 2015-04-27 14:45:52
Malyhon: 金币+8, 翻译EPI+1, ★★★★★最佳答案 2015-04-27 14:45:52
| 2.7. 通过光波导模式谱(OWLS)的血清吸附试验。 通过光波导模式谱在未修饰和各种修饰的PHBV膜上进行了非特异性蛋白由血清的吸附试验。30,33总而言之, 2wt%的PHBV旋涂到波导芯片上,并按2.1节中的叙述进行随后的表面修饰。为与MAL-PEG接枝的PHBV膜的性质进行比较,通过NH3-等离子体处理过的PHBV与甲氧基聚(乙二醇)丙酸(分子量2 or 5 kDa NHS-PEG-OCH3, Jenkem技术公司,中国,北京)的2mM的N-羟基琥珀酰亚胺酯于HEPES - NaCl缓冲液(10mM HEPES, 150mM NaCl, pH7.2)中进行反应1小时制备了CH3OPEG-接枝的PHBV膜(PHBV-PEGOCH3)。 |
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