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In time course experiments, incubations were carried out with 0.7 g/L concentration of I/II in fermentation medium and the level of transformation was monitored by HPLC at every 24 h for 6 days. Quantitation and the levels of different metabolites formed were determined on the basis of area under the respective peak and compared with the standard graphs obtained for individual metabolite by injecting the known amounts of each metabolite. All the experiments were carried out in triplicates and under identical conditions. |
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bxzc123(RXMCDM代发): 金币+40, 多谢应助! 2015-09-02 23:28:50
bxzc123(RXMCDM代发): 金币+40, 多谢应助! 2015-09-02 23:28:50
| 在时程实验,用含有I/II浓度为0.7g/L的发酵培养基进行培养,并通过HPLC每24小时监测转化水平,共监测6天。基于各自峰下面积进行定量并测定所形成的不同代谢物的水平,峰下面积与个别代谢物的标准曲线(峰下面积)相比较。标准曲线是通过注入已知量的每种代谢物获得的。所有实验均一式三份,并在相同的条件下进行。 |
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