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zsw2003

金虫 (正式写手)

[求助] 关于EI主题词检索 已有2人参与

投一篇EI收录的期刊,要求提供三个EI主题词,请问怎么检索?

我们单位没有EI的权限,不知有没有虫友愿意帮忙检索一下?金币感谢
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kinglai07

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★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ★ ...
zsw2003: 金币+100, ★★★★★最佳答案 2015-03-29 16:44:15
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7楼2015-03-29 16:03:02
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kinglai07

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感谢参与,应助指数 +1
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2楼2015-03-29 14:31:44
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zsw2003

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引用回帖:
2楼: Originally posted by kinglai07 at 2015-03-29 14:31:44
我可以帮你检索,但是你说的主题词是什么?关键词?

Research on Quantitation of Almond Protein in Almond Protein Beverage

Abstract:The aim of the research was to develop competitive enzyme linked immunosorbent assay (ELISA) for quantitation of almond protein in almond protein beverage. We applied two-dimensional electrophoresis (2-DE) to compare the differences between almond and apricot kernel to search for almond-specific proteins. A Pru-1 subunit fragment with a mass of 27 kDa and pI (isoelectric point) of 5.5-8.0 was found, extracted, purified and used as antigen to prepare a specific monoclonal antibody. Using the monoclonal antibody and almond soluble protein as standard, an optimized ELISA method was established with IC50 of 3.3 μg/mL and linear detection range of 5.0 to 200 μg/mL (R2=0.99). The assay did not register cross-reactivity with tested apricot kernels and other edible plant seeds. The sample extracting solution was 7M urea with 0.5%DTT. The limit of detection (LOD) was 100 μg/g, with relative standard deviation less than 10%. The average recovery rates were 97%, 93%and 84% under the sterilization condition of pasteurization, ultra high temperature treated and autoclaved sterilization, respectively.

Key words: Almond; Protein beverage; ELISA; Quantitation
3楼2015-03-29 14:40:33
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zsw2003

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杂志社里面的要求:请自行搜索EI主题词并采用,每篇文中要求采用至少3个EI要求的主题词
4楼2015-03-29 14:43:46
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