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zsw2003金虫 (正式写手)
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投一篇EI收录的期刊，要求提供三个EI主题词，请问怎么检索？ 我们单位没有EI的权限，不知有没有虫友愿意帮忙检索一下？金币感谢 |
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lilyjr
新虫 (小有名气)
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11楼2015-09-15 14:18:02
感谢参与，应助指数 +1
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2楼2015-03-29 14:31:44
zsw2003
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Research on Quantitation of Almond Protein in Almond Protein Beverage Abstract：The aim of the research was to develop competitive enzyme linked immunosorbent assay (ELISA) for quantitation of almond protein in almond protein beverage. We applied two-dimensional electrophoresis (2-DE) to compare the differences between almond and apricot kernel to search for almond-specific proteins. A Pru-1 subunit fragment with a mass of 27 kDa and pI (isoelectric point) of 5.5-8.0 was found, extracted, purified and used as antigen to prepare a specific monoclonal antibody. Using the monoclonal antibody and almond soluble protein as standard, an optimized ELISA method was established with IC50 of 3.3 μg/mL and linear detection range of 5.0 to 200 μg/mL (R2=0.99). The assay did not register cross-reactivity with tested apricot kernels and other edible plant seeds. The sample extracting solution was 7M urea with 0.5%DTT. The limit of detection (LOD) was 100 μg/g, with relative standard deviation less than 10%. The average recovery rates were 97%, 93%and 84% under the sterilization condition of pasteurization, ultra high temperature treated and autoclaved sterilization, respectively. Key words: Almond; Protein beverage; ELISA; Quantitation |
3楼2015-03-29 14:40:33
zsw2003
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4楼2015-03-29 14:43:46